Analysis of inflammation and bone remodeling of atmospheric plasma therapy in experimental periodontitis

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of periodontal research Pub Date : 2024-04-02 DOI:10.1111/jre.13248

Ildamara Canoa de Oliveira, Mariana Zanco, Juliana Lopes, Milena Paloma Sambo, Thiago Antonio Moretti de Andrade, Gláucia Maria Tech dos Santos, Maira Felonato, Milton Santamaria-Jr

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Abstract

Background and Objective

The biological effects of atmospheric plasma (cold plasma) show its applicability for controlling the etiological factors that involve tissue repair. Thus, the study evaluated the effect of atmospheric plasma therapy in the control of tissue inflammation and bone remodeling in experimental periodontitis.

Methods

Fifty-six rats were subjected to ligation in the cervical region of the first maxillary molars (8 weeks). The animals were divided into two groups (n = 28): periodontitis without treatment group (P group), and periodontitis with atmospheric plasma treatment group (P + AP group). Tissue samples were collected at 2 and 4 weeks after treatment to analyze the inflammation and bone remodeling by biochemical, histomorphometric, and immunohistochemical analyses.

Results

Inflammatory infiltration in the gingival and periodontal ligament was lower in the P + AP group than in the P group (p < .05). The MPO and NAG levels were higher in the P + AP group compared to P group (p < .05). At 4 weeks, the TNF-α level was lower and the IL-10 level was higher in the P + AP group compared to P group (p < .05). In the P + AP group, the IL-1β level increased in the second week and decreased in the fourth week (p < .05), the number of blood vessels was high in the gingival and periodontal ligament in the second and fourth week (p < .05); and the number of fibroblasts in the gingival tissue was low in the fourth week, and higher in the periodontal tissue in both period (p < .05). Regarding bone remodeling, the RANK and RANKL levels decreased in the P + AP group (p < .05). The OPG level did not differ between the P and P + AP groups (p > .05), but decreased from the second to the fourth experimental week in P + AP group (p < .05).

Conclusions

The treatment of experimental periodontitis with atmospheric plasma for 4 weeks modulated the inflammatory response to favor the repair process and decreased the bone resorption biomarkers, indicating a better control of bone remodeling in periodontal disease.

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大气等离子体疗法在实验性牙周炎中的炎症和骨重塑分析

背景和目的大气等离子体（冷等离子体）的生物效应表明，它适用于控制涉及组织修复的病因。因此，本研究评估了常压等离子体疗法在控制实验性牙周炎的组织炎症和骨重塑方面的效果。动物分为两组（n = 28）：无治疗的牙周炎组（P 组）和有大气血浆治疗的牙周炎组（P + AP 组）。在治疗后 2 周和 4 周采集组织样本，通过生化、组织形态学和免疫组化分析来分析炎症和骨重塑情况。结果P + AP 组牙龈和牙周韧带的炎症浸润低于 P 组（p < .05）。与 P 组相比，P + AP 组的 MPO 和 NAG 水平更高（p < .05）。4 周时，与 P 组相比，P + AP 组的 TNF-α 水平较低，IL-10 水平较高（p < .05）。在 P + AP 组中，IL-1β 水平在第二周升高，在第四周降低（p < .05）；在第二周和第四周，牙龈和牙周韧带中的血管数量较多（p < .05）；在第四周，牙龈组织中的成纤维细胞数量较少，而在这两个时期，牙周组织中的成纤维细胞数量较多（p < .05）。在骨重塑方面，P + AP 组的 RANK 和 RANKL 水平下降（p < .05）。结论用大气血浆治疗实验性牙周炎 4 周，调节了炎症反应，有利于修复过程，并降低了骨吸收生物标志物，表明对牙周病的骨重塑有更好的控制作用。

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来源期刊

Journal of periodontal research 医学-牙科与口腔外科

CiteScore

6.90

自引率

5.70%

发文量

103

审稿时长

6-12 weeks

期刊介绍： The Journal of Periodontal Research is an international research periodical the purpose of which is to publish original clinical and basic investigations and review articles concerned with every aspect of periodontology and related sciences. Brief communications (1-3 journal pages) are also accepted and a special effort is made to ensure their rapid publication. Reports of scientific meetings in periodontology and related fields are also published. One volume of six issues is published annually.