ETV2 induces endothelial, but not hematopoietic, lineage specification in birds.

IF 3.3 2区生物学 Q1 BIOLOGY Life Science Alliance Pub Date : 2024-04-03 DOI:10.26508/lsa.202402694

Wei Weng, Yuan Deng, Ruslan Deviatiiarov, Sofiane Hamidi, Eriko Kajikawa, Oleg Gusev, Hiroshi Kiyonari, Guojie Zhang, Guojun Sheng

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Abstract

Cardiovascular system develops from the lateral plate mesoderm. Its three primary cell lineages (hematopoietic, endothelial, and muscular) are specified by the sequential actions of conserved transcriptional factors. ETV2, a master regulator of mammalian hemangioblast development, however, is absent in the chicken genome and acts downstream of NPAS4L in zebrafish. Here, we investigated the epistatic relationship between NPAS4L and ETV2 in avian hemangioblast development. We showed that ETV2 is deleted in all 363 avian genomes analyzed. Mouse ETV2 induced LMO2, but not NPAS4L or SCL, expression in chicken mesoderm. Squamate (lizards, geckos, and snakes) genomes contain both NPAS4L and ETV2 In Madagascar ground gecko, both genes were expressed in developing hemangioblasts. Gecko ETV2 induced only LMO2 in chicken mesoderm. We propose that both NPAS4L and ETV2 were present in ancestral amniote, with ETV2 acting downstream of NPAS4L in endothelial lineage specification. ETV2 may have acted as a pioneer factor by promoting chromatin accessibility of endothelial-specific genes and, in parallel with NPAS4L loss in ancestral mammals, has gained similar function in regulating blood-specific genes.

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ETV2 可诱导鸟类内皮细胞而非造血细胞系的形成。

心血管系统由侧板中胚层发育而成。它的三个主要细胞系（造血细胞系、内皮细胞系和肌肉细胞系）是由保守的转录因子依次作用而形成的。ETV2 是哺乳动物血管母细胞发育的主要调控因子，但在鸡基因组中并不存在，在斑马鱼中则作用于 NPAS4L 的下游。在这里，我们研究了 NPAS4L 和 ETV2 在禽类血管母细胞发育过程中的表观关系。我们发现，在分析的所有 363 个禽类基因组中，ETV2 都被删除了。小鼠 ETV2 能诱导鸡中胚层中 LMO2 的表达，但不能诱导 NPAS4L 或 SCL 的表达。有鳞类（蜥蜴、壁虎和蛇）基因组中同时含有 NPAS4L 和 ETV2 在马达加斯加地壁虎中，这两个基因都在发育中的血管母细胞中表达。壁虎的 ETV2 只诱导鸡中胚层中的 LMO2。我们认为，NPAS4L和ETV2都存在于祖先羊膜动物中，ETV2在NPAS4L的下游作用于内皮细胞系的分化。ETV2可能通过促进内皮特异性基因染色质的可及性而充当先驱因子，并且随着NPAS4L在祖先哺乳动物中的缺失，ETV2在调控血液特异性基因方面也获得了类似的功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Life Science Alliance Agricultural and Biological Sciences-Plant Science

CiteScore

5.80

自引率

2.30%

发文量

241

审稿时长

10 weeks

期刊介绍： Life Science Alliance is a global, open-access, editorially independent, and peer-reviewed journal launched by an alliance of EMBO Press, Rockefeller University Press, and Cold Spring Harbor Laboratory Press. Life Science Alliance is committed to rapid, fair, and transparent publication of valuable research from across all areas in the life sciences.