Influence of vitamins and food on the fluorescence spectrum of human urine

IF 2.2 3区 医学 Q2 DERMATOLOGY Lasers in Surgery and Medicine Pub Date : 2024-04-11 DOI:10.1002/lsm.23785
Praveen Chalissery, Christian Homann PhD, Herbert Stepp PhD, Maximilian Eisel PhD, Maximilian Aumiller PhD, Adrian Rühm PhD, Alexander Buchner MD, Ronald Sroka PhD
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Abstract

Objectives

Fluorescence spectroscopy of human urine is a method with the potential to gain importance as a diagnostic tool in the medical field, e.g., for measuring Coproporphyrin III (CPIII) as an indicator of cancer and acute types of porphyria. Food can change human urine's color, which could influence the urine fluorescence spectrum and the detection of CPIII in urine. To determine if there is a noticeable influence on the urine fluorescence spectrum or on the detection of CPIII in urine, 16 vitamin supplements, and three food items were tested. Such investigation may also prevent false interpretation of measured data.

Methods

Urine samples were collected before and after (overnight, ca. 8 h) intake of each test substance. Samples were investigated by fluorescence spectrum analysis. At excitation wavelengths from 300 to 500 nm and emission wavelengths from 400 to 700 nm excitation-emission-matrices were measured. Data obtained from urine before intake were compared to the data from overnight urine. Furthermore, the investigation of any interference with the CPIII concentration was performed at an excitation wavelength of 407 ± 3 nm and emission wavelengths of 490−800 nm.

Results

Only vitamin B2, but none of the other tested substances, showed noticeable influence on the urine fluorescence spectrum. None of the tested substances showed noticeable interference with the recovery rate of CPIII.

Conclusions

The correct interpretation of measured data by fluorescence spectroscopy is possible with the exception if vitamin B2 supplementation was performed; thus, the consumption of vitamin B2 supplements before fluorescence testing of the patient's urine should be avoided and/or must be requested. CPIII concentrations could reliably be measured in all cases.

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维生素和食物对人体尿液荧光光谱的影响
目的人体尿液荧光光谱法是一种有可能在医学领域作为重要诊断工具的方法,例如用于测量作为癌症和急性卟啉症指标的铜卟啉 III(CPIII)。食物会改变人体尿液的颜色,从而影响尿液荧光光谱和尿液中 CPIII 的检测。为了确定对尿液荧光光谱或尿液中 CPIII 的检测是否有明显影响,我们对 16 种维生素补充剂和 3 种食物进行了检测。方法在摄入每种测试物质前后(过夜,约 8 小时)收集尿液样本。通过荧光光谱分析对样本进行调查。激发波长为 300 至 500 nm,发射波长为 400 至 700 nm。将摄入前尿液的数据与隔夜尿液的数据进行比较。此外,在激发波长为 407 ± 3 nm 和发射波长为 490-800 nm 时,对 CPIII 浓度的干扰进行了调查。结论通过荧光光谱法可以正确解释所测得的数据,但补充维生素 B2 的情况除外;因此,在对患者的尿液进行荧光检测之前,应避免服用维生素 B2 补充剂,并且/或者必须要求服用维生素 B2 补充剂。在所有情况下都能可靠地测量出 CPIII 的浓度。
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来源期刊
CiteScore
5.40
自引率
12.50%
发文量
119
审稿时长
1 months
期刊介绍: Lasers in Surgery and Medicine publishes the highest quality research and clinical manuscripts in areas relating to the use of lasers in medicine and biology. The journal publishes basic and clinical studies on the therapeutic and diagnostic use of lasers in all the surgical and medical specialties. Contributions regarding clinical trials, new therapeutic techniques or instrumentation, laser biophysics and bioengineering, photobiology and photochemistry, outcomes research, cost-effectiveness, and other aspects of biomedicine are welcome. Using a process of rigorous yet rapid review of submitted manuscripts, findings of high scientific and medical interest are published with a minimum delay.
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