Development of Twenty Novel Polymorphic Microsatellite Markers and Their Application in Population Genetic Studies of Konosirus punctatus

Thalassas: An International Journal of Marine Sciences Pub Date : 2024-04-16 DOI:10.1007/s41208-024-00712-3

Zengliang Miao, Xun Jin, Shiyi Chen, Kun Zhang, Jiasheng Li, Ying Peng, Wenhua Huang, Xudong Liang, Haodi Shen, Yifan Liu, Bingjian Liu

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Abstract

Konosirus punctatus is an important fishery resource which is distributed from the Indian Ocean to the northwest Pacific. To study the genetic diversity of K. punctatus, we designed forty microsatellite markers based on Illumina and PacBio sequencing data. Twenty of them (50%) were successfully amplified and exhibited polymorphism which were selected for population genetic analyses in three K. punctatus populations from China (Xiamen, Zhoushan, and Laizhou). The number of alleles per locus ranged from 8 to 15, and the values of expected heterozygosity (He) and observed heterozygosity (Ho) were 0.454 ~ 0.918 (mean = 0.775) and 0.125 ~ 0.958 (mean = 0.706), respectively. The F_ST showed that the three geographic populations were moderately differentiated, while Structure and PCoA analysis showed that there was no significant differentiation between pairwise populations. These microsatellite markers will provide a basis for the study of the population genetics of K. punctatus.

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开发 20 个新型多态性微卫星标记及其在 Konosirus punctatus 群体遗传研究中的应用

Konosirus punctatus是一种重要的渔业资源，分布于印度洋到西北太平洋。为了研究K. punctatus的遗传多样性，我们根据Illumina和PacBio测序数据设计了40个微卫星标记。其中20个标记（50%）被成功扩增并表现出多态性，我们选择了这些标记在中国的三个K. punctatus种群（厦门、舟山和莱州）中进行种群遗传分析。每个位点的等位基因数为 8 至 15 个，预期杂合度（He）和观察杂合度（Ho）分别为 0.454 ~ 0.918（平均值 = 0.775）和 0.125 ~ 0.958（平均值 = 0.706）。FST 分析表明，三个地理种群之间存在中度分化，而结构和 PCoA 分析表明，成对种群之间没有显著分化。这些微卫星标记将为K. punctatus的种群遗传学研究提供依据。

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Thalassas: An International Journal of Marine Sciences

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