A study on Wolbachia-dengue-carrying Aedes mosquitoes (diptera: culicidae) focuses on the sustainability and frequency of Wolbachia in high-rise buildings in Selangor, Malaysia

IF 1.3 4区农林科学 Q2 ENTOMOLOGY Applied Entomology and Zoology Pub Date : 2024-04-01 DOI:10.1007/s13355-024-00870-z

Muhammad Aidil Roslan, Romano Ngui, Mas-Azlindah-Ayu Abd Karim, Ummi Syafiqah Rosmini, Poon Soon Ong, Mas Ayu Ahmad, Yvonne Ai Lian Lim, Wan Yusoff Wan Sulaiman

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Abstract

The challenge of dengue control due to the unavailability of a specific medication stresses the importance of releasing Wolbachia-carrying mosquitoes through vector control programs. This study investigated the sustainability and frequency of Wolbachia in Wolbachia-dengue-carrying mosquitoes in two dengue hotspot localities in Selangor. A modified sticky ovitrap was used to collect adult mosquitoes in two Wolbachia-releasing areas in Selangor, Kelana Puteri and Kelana D'Putera condominiums. All mosquito samples were subjected to PCR using wsp-specific primers for Wolbachia detection. Dengue virus was detected using RT-PCR, followed by multiplex-PCR. Out of the 80 Aedes spp. collected, Ae. aegypti was the most predominant species. More than one-third of Ae. aegypti were positive for Wolbachia, with 22.9% being superinfected with both Wolbachia A and B strains. About 61.4% of the species were uninfected with Wolbachia. Ae. aegypti carrying the Wolbachia A strain was also identified, which has previously never been reported. This strain was similar to the one found naturally in Ae. albopictus. None of the Ae. aegypti and Ae. albopictus were positive for dengue virus. This study could serve as a model for local researchers or health authorities to design and plan an effective field release and monitoring of Wolbachia-infected mosquitoes.

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关于携带沃尔巴克氏体的伊蚊（双翅目：伊蚊科）的研究，重点是马来西亚雪兰莪州高层建筑中沃尔巴克氏体的可持续性和频率

摘要由于没有特效药物，登革热控制面临挑战，这就强调了通过病媒控制项目释放携带沃尔巴克氏体蚊子的重要性。本研究调查了雪兰莪州两个登革热热点地区携带沃尔巴克氏体蚊子中沃尔巴克氏体的可持续性和频率。研究人员在雪兰莪州的两个沃尔巴克氏体释放区（Kelana Puteri和Kelana D'Putera公寓）使用改良的粘性誘捕器收集成蚊。所有蚊子样本都使用 wsp 特异引物进行 PCR 检测。登革热病毒的检测采用 RT-PCR，然后是多重 PCR。在收集到的 80 种伊蚊中，埃及伊蚊是最主要的种类。超过三分之一的埃及伊蚊对沃尔巴克氏体呈阳性反应，其中 22.9% 的埃及伊蚊同时被沃尔巴克氏体 A 和 B 株超级感染。约 61.4% 的埃及姬蜂未感染沃尔巴克氏体。此外，还发现埃及蚁携带沃尔巴克氏体 A 株，这在以前从未报道过。这种菌株与在白纹伊蚊中自然发现的菌株相似。埃及蚁和白纹伊蚊均未对登革热病毒呈阳性反应。这项研究可作为当地研究人员或卫生当局设计和规划有效的野外释放和监测沃尔巴奇感染蚊子的范例。

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来源期刊

Applied Entomology and Zoology 生物-昆虫学

CiteScore

2.70

自引率

7.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Entomology and Zoology publishes articles concerned with applied entomology, applied zoology, agricultural chemicals and pest control in English. Contributions of a basic and fundamental nature may be accepted at the discretion of the Editor. Manuscripts of original research papers, technical notes and reviews are accepted for consideration. No manuscript that has been published elsewhere will be accepted for publication.