A comparative study on targeted gene expression in zebrafish and its gill cell line exposed to chlorpyrifos

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-04-08 DOI:10.1007/s11626-024-00892-5

Abdul Wazith M. J., Taju G., Abdul Majeed S., Mithra S., Nafeez Ahmed A., Badhusha A., Rajkumar V., Sahul Hameed A. S.

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Abstract

Chlorpyrifos (CPF) is an organophosphorus-based insecticide, which is known to pose a serious risk to aquatic animals. However, the mechanisms of CPF toxicity in animals still remain unclear. The present investigation aimed to compare the potential effects of CPF in zebrafish (Danio rerio) and its gill cell line (DrG cells). Based on the in vivo study, the LC₅₀ was calculated as 18.03 µg/L and the chronic toxic effect of CPF was studied by exposing the fish to 1/10th (1.8 µg/L) and 1/5th (3.6 µg/L) of the LC₅₀ value. Morphological changes were observed in fish and DrG cells which were exposed to sublethal concentrations of CPF. The results of MTT and NR assays showed significant decline in the survival of cells exposed to CPF at 96 h. The production of reactive oxygen species in DrG cells and expression levels of antioxidant markers, inflammatory response genes (cox2a and cox2b), cyp1a, proapoptotic genes (bax), antiapoptotic gene (bcl2), apoptotic genes (cas3 and p53), and neuroprotective gene (ache) were determined in vivo using zebrafish and in vitro using DrG cells after exposure to CPF. Significant changes were found in the ROS production (DrG cells) and in the expression of inflammatory, proapoptotic, and apoptotic genes. This study showed that DrG cells are potential alternative tools to replace the use of whole fish for toxicological studies.

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暴露于毒死蜱的斑马鱼及其鳃细胞系中靶基因表达的比较研究

毒死蜱（CPF）是一种有机磷类杀虫剂，已知会对水生动物造成严重危害。然而，毒死蜱在动物体内的毒性机制仍不清楚。本研究旨在比较 CPF 对斑马鱼（Danio rerio）及其鳃细胞系（DrG 细胞）的潜在影响。根据体内研究，计算出半数致死浓度为 18.03 微克/升，并通过让鱼类接触半数致死浓度的 1/10 (1.8 微克/升) 和 1/5 (3.6 微克/升) 来研究氯化石蜡的慢性毒性效应。鱼类和 DrG 细胞暴露于亚致死浓度的氯化石蜡后，形态发生了变化。MTT 和 NR 检测结果表明，暴露于氯化石蜡的细胞在 96 小时后存活率显著下降。用斑马鱼在体内和用 DrG 细胞在体外测定了暴露于 CPF 后 DrG 细胞中活性氧的产生以及抗氧化标志物、炎症反应基因（cox2a 和 cox2b）、cyp1a、促凋亡基因（bax）、抗凋亡基因（bcl2）、凋亡基因（cas3 和 p53）和神经保护基因（ache）的表达水平。结果发现，ROS 的产生（DrG 细胞）以及炎症基因、促凋亡基因和凋亡基因的表达均发生了显著变化。这项研究表明，DrG 细胞是替代整鱼进行毒理学研究的潜在工具。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.