Antibacterial, antibiofilm, and antivirulence potential of the main diterpenes from Copaifera spp. oleoresins against multidrug‐resistant bacteria

Naunyn-schmiedebergs Archives of Pharmacology Pub Date : 2024-04-15 DOI:10.1007/s00210-024-03077-9

L. F. Leandro, T. S. Moraes, J. L. Damasceno, R. C. S. Veneziani, S. R. Ambrosio, J. K. Bastos, M. B. Santiago, R. S. Pedroso, C. H. G. Martins

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Abstract

To evaluate the antibacterial, antibiofilm and antivirulence potential of the main diterpenes from Copaifera spp. oleoresins against multidrug‐resistant (MDR) bacteria. Antimicrobial assays included determination of the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), Minimum Inhibitory Concentration of Biofilm (MICB₅₀), as well as synergistic and antivirulence assays for eight diterpenes against MDR. The tests revealed that two diterpenes (named 1 and 5) showed the best results, with MIC and MBC between 12.5 and 50 μg/mL against most MDR bacteria. These diterpenes exhibited promising MICB₅₀ in concentration between 3.12–25 μg/mL but showed no synergistic antimicrobial activity. In the assessment of antivirulence activity, diterpenes 1 and 5 inhibited only one of the virulence factors evaluated (Dnase) produced by some strains of S. aureus at subinhibitory concentration (6.25 μg/mL). Results obtained indicated that diterpenes isolated from Copaifera oleoresin plays an important part in the search of new antibacterial and antibiofilm agents that can act against MDR bacteria.

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科帕菲拉属植物油树脂中的主要二萜对耐多药细菌的抗菌、抗生物膜和抗病毒潜力

评估科帕菲拉属植物油树脂中的主要二萜对耐多药（MDR）细菌的抗菌、抗生物膜和抗病毒潜力。抗菌试验包括测定八种二萜对 MDR 的最低抑菌浓度（MIC）、最低杀菌浓度（MBC）、最低生物膜抑菌浓度（MICB50），以及协同和抗病毒试验。测试结果表明，两种二萜（分别命名为 1 和 5）效果最佳，对大多数 MDR 细菌的 MIC 和 MBC 在 12.5 和 50 μg/mL 之间。这些二萜在 3.12-25 μg/mL 的浓度范围内显示出良好的 MICB50，但没有显示出协同抗菌活性。在抗病毒活性评估中，二萜 1 和 5 仅在亚抑制浓度（6.25 μg/mL）下抑制了一些金黄色葡萄球菌菌株产生的一种毒力因子（Dnase）。研究结果表明，从 Copaifera 油树脂中分离出的二萜类化合物在寻找新的抗菌和抗生物膜剂方面发挥着重要作用，这些新的抗菌和抗生物膜剂可对抗耐药菌。

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Naunyn-schmiedebergs Archives of Pharmacology

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