Effect of Silver-Graphene Oxide-Cobalt Oxide Nanocomposite on Cytotoxic Levels in MRC-5 and HepG2 Cell Lines and Molecular Docking Studies

IF 3.6 4区化学 Q2 CHEMISTRY, INORGANIC & NUCLEAR Journal of Cluster Science Pub Date : 2024-04-06 DOI:10.1007/s10876-024-02604-1

Rahsan Ilikci-Sagkan, Erman Salih Istifli, Recep Liman, Keziban Atacan, Salih Zeki Bas, Mustafa Ozmen

{"title":"Effect of Silver-Graphene Oxide-Cobalt Oxide Nanocomposite on Cytotoxic Levels in MRC-5 and HepG2 Cell Lines and Molecular Docking Studies","authors":"Rahsan Ilikci-Sagkan, Erman Salih Istifli, Recep Liman, Keziban Atacan, Salih Zeki Bas, Mustafa Ozmen","doi":"10.1007/s10876-024-02604-1","DOIUrl":null,"url":null,"abstract":"<div>The cytotoxic properties of cobalt oxide (Co3O4) nanoparticles (NPs), in addition to graphene oxide (GO)-Co3O4 and silver (Ag)-GO-Co3O4 nanocomposites (NCs), were evaluated against both human healthy lung fibroblast (MRC-5) and hepatocellular carcinoma (HepG2) cell lines utilizing the XTT assay. The investigation revealed that synthesized Co3O4 NPs and NCs (GO-Co3O4 and Ag-GO-Co3O4) elicited significant cytotoxic responses in MRC-5 and HepG2 cell lines in a concentration-dependent manner. Through molecular docking analyses, it was observed that all fabricated nanomaterials exhibited DNA recognition via minor groove binding, with molecular affinities ranging from − 4.82 to -11.66 kcal/mol. Furthermore, the docking outcomes illustrated that the angular conformations of GO-Co3O4 and Ag-GO-Co3O4 conferred ‘shape-selective’ characteristics as DNA minor groove binders, leading to heightened cytotoxicity, particularly in the HepG2 cell line compared to the normal MRC-5 cell line.</div>","PeriodicalId":618,"journal":{"name":"Journal of Cluster Science","volume":"35 5","pages":"1481 - 1491"},"PeriodicalIF":3.6000,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10876-024-02604-1.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cluster Science","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s10876-024-02604-1","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}

引用次数: 0

Abstract

The cytotoxic properties of cobalt oxide (Co₃O₄) nanoparticles (NPs), in addition to graphene oxide (GO)-Co₃O₄ and silver (Ag)-GO-Co₃O₄ nanocomposites (NCs), were evaluated against both human healthy lung fibroblast (MRC-5) and hepatocellular carcinoma (HepG2) cell lines utilizing the XTT assay. The investigation revealed that synthesized Co₃O₄ NPs and NCs (GO-Co₃O₄ and Ag-GO-Co₃O₄) elicited significant cytotoxic responses in MRC-5 and HepG2 cell lines in a concentration-dependent manner. Through molecular docking analyses, it was observed that all fabricated nanomaterials exhibited DNA recognition via minor groove binding, with molecular affinities ranging from − 4.82 to -11.66 kcal/mol. Furthermore, the docking outcomes illustrated that the angular conformations of GO-Co₃O₄ and Ag-GO-Co₃O₄ conferred ‘shape-selective’ characteristics as DNA minor groove binders, leading to heightened cytotoxicity, particularly in the HepG2 cell line compared to the normal MRC-5 cell line.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

银-氧化石墨烯-氧化钴纳米复合材料对 MRC-5 和 HepG2 细胞系细胞毒性水平的影响及分子对接研究

摘要利用 XTT 试验评估了氧化钴（Co3O4）纳米颗粒（NPs）以及氧化石墨烯（GO）-Co3O4 和银（Ag）-GO-Co3O4 纳米复合材料（NCs）对人类健康肺成纤维细胞（MRC-5）和肝细胞癌（HepG2）细胞系的细胞毒性。研究结果表明，合成的 Co3O4 NPs 和 NCs（GO-Co3O4 和 Ag-GO-Co3O4）以浓度依赖的方式在 MRC-5 和 HepG2 细胞系中引起了显著的细胞毒性反应。通过分子对接分析发现，所有制备的纳米材料都能通过次凹槽结合识别 DNA，分子亲和力范围为 - 4.82 至 -11.66 kcal/mol。此外，对接结果表明，GO-Co3O4 和 Ag-GO-Co3O4 的角构象具有作为 DNA 小沟结合剂的 "形状选择性 "特征，从而提高了细胞毒性，特别是在 HepG2 细胞系中的细胞毒性高于正常的 MRC-5 细胞系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Journal of Cluster Science 化学-无机化学与核化学

CiteScore

6.70

自引率

0.00%

发文量

166

审稿时长

3 months

期刊介绍： The journal publishes the following types of papers: (a) original and important research; (b) authoritative comprehensive reviews or short overviews of topics of current interest; (c) brief but urgent communications on new significant research; and (d) commentaries intended to foster the exchange of innovative or provocative ideas, and to encourage dialogue, amongst researchers working in different cluster disciplines.