Designing a Novel Multi-Epitope Peptide as a Potential Serodiagnosis Marker for the Diagnosis of Acinetobacter baumannii: An In silico Approach

IF 0.5 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS Current Proteomics Pub Date : 2024-04-02 DOI:10.2174/0115701646297689240325062145

Maryam Rezaee, Mohsen Mohammadi, Amir Savardashtaki, Mohammad Reza Rahbar, Navid Nezafat

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Abstract

Background: Acinetobacter baumannii is an opportunistic pathogen that causes many infections, including nosocomial infections; this bacterium has a high mortality rate among other bacteria. A. baumannii has an elastic genome that changes rapidly when exposed to harsh environmental conditions, leading to widespread bacterial resistance to various disinfectants and antibiotics. The high ability of bacteria to bind to all surfaces and survive in different conditions has caused the spread of bacteria in various environments. Rapid detection is very important in preventing the spread and even treatment of the infection. Methods: Currently, the Polymerase Chain Reaction (PCR) method is the only effective method used for diagnosis, which has some pros and cons. Results and Conclusion: This study aimed to design a new recombinant multi-epitope protein from Acinetobacter baumannii that can be used in ELISA for rapid diagnosis. The unique feature of this study from others is the use of patient serum for antibody monitoring.

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设计一种新型多表位肽作为诊断鲍曼不动杆菌的潜在血清诊断标记物：硅学方法

背景：鲍曼不动杆菌（Acinetobacter baumannii）是一种机会性病原体，可引起多种感染，包括医院内感染；在其他细菌中，这种细菌的死亡率很高。鲍曼不动杆菌的基因组具有弹性，当暴露在恶劣的环境条件下时，基因组会迅速发生变化，从而导致细菌对各种消毒剂和抗生素产生广泛的抗药性。细菌具有很强的与各种表面结合的能力，并能在不同的条件下存活，这就造成了细菌在各种环境中的传播。快速检测对于防止感染扩散甚至治疗都非常重要。方法：目前，聚合酶链反应（PCR）法是唯一有效的诊断方法，但这种方法有利有弊。结果与结论：本研究旨在设计一种新的重组鲍曼不动杆菌多表位蛋白，可用于 ELISA 快速诊断。与其他研究相比，本研究的独特之处在于使用患者血清进行抗体监测。

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来源期刊

Current Proteomics BIOCHEMICAL RESEARCH METHODS-BIOCHEMISTRY & MOLECULAR BIOLOGY

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>0 weeks

期刊介绍： Research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed in-depth/mini review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry. Current Proteomics publishes in-depth/mini review articles in all aspects of the fast-expanding field of proteomics. All areas of proteomics are covered together with the methodology, software, databases, technological advances and applications of proteomics, including functional proteomics. Diverse technologies covered include but are not limited to: Protein separation and characterization techniques 2-D gel electrophoresis and image analysis Techniques for protein expression profiling including mass spectrometry-based methods and algorithms for correlative database searching Determination of co-translational and post- translational modification of proteins Protein/peptide microarrays Biomolecular interaction analysis Analysis of protein complexes Yeast two-hybrid projects Protein-protein interaction (protein interactome) pathways and cell signaling networks Systems biology Proteome informatics (bioinformatics) Knowledge integration and management tools High-throughput protein structural studies (using mass spectrometry, nuclear magnetic resonance and X-ray crystallography) High-throughput computational methods for protein 3-D structure as well as function determination Robotics, nanotechnology, and microfluidics.