Cu-MOF/Aptamer/Magnetic Bead Sensor for Detection of Breast Cancer Biomarkers HER2 and ER

IF 8.3 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY ACS Applied Materials & Interfaces Pub Date : 2024-04-12 DOI:10.1021/acsanm.4c01671

Xiaofang Liu, Ya Zhang, Ning Li, Zhihao Yang, Siyi Yang, Jiawei Li, Jian Chen*, Danqun Huo* and Changjun Hou*,

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Abstract

Cancer markers are used for early cancer detection and follow-up monitoring so that patients receive the most appropriate treatment to alleviate suffering and prolong life. Herein, a metal–organic framework (MOF) combined with magnetic beads (MB) is proposed as a nanosensor for detecting breast cancer biomarkers based on magnetic enrichment and separation. With the porous structure of Cu-MOF, a large number of aptamers are adsorbed inside the pore to specifically capture the antigen. Then, Cu-MOF@antigen is enriched and separated by MB modified with the corresponding antibody. Supernatant Cu-MOF and Cu-MOF@antigen@MB are used as colorimetric, fluorescent, and electrochemical sensing elements for the quantitative detection of HER2 and ER. The detection ranges for HER2 and ER are 4.5 fg/mL–20 ng/mL and 10 fg/mL–20 ng/mL, respectively. It also demonstrated good performance in the detection of human serum samples, with recoveries ranging from 89.00 to 107.57%. This nanosensor has promising selectivity, stability, and practical applicability, providing the possibility for its in-depth development.

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用于检测乳腺癌生物标记物 HER2 和 ER 的 Cu-MOF/Aptamer/Magnetic Bead 传感器

癌症标志物用于早期癌症检测和后续监测，以便患者接受最适当的治疗，减轻痛苦并延长生命。本文提出了一种金属有机框架（MOF）与磁珠（MB）相结合的纳米传感器，用于检测基于磁富集和分离的乳腺癌生物标记物。利用 Cu-MOF 的多孔结构，大量适配体被吸附在孔内，从而特异性地捕获抗原。然后，Cu-MOF@抗原被相应抗体修饰的 MB 富集和分离。上清液 Cu-MOF 和 Cu-MOF@antigen@MB 可用作比色、荧光和电化学传感元件，用于定量检测 HER2 和 ER。HER2 和 ER 的检测范围分别为 4.5 fg/mL-20 ng/mL 和 10 fg/mL-20 ng/mL。它在人血清样品的检测中也表现出良好的性能，回收率在 89.00% 至 107.57% 之间。该纳米传感器具有良好的选择性、稳定性和实用性，为其深入开发提供了可能。

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来源期刊

ACS Applied Materials & Interfaces 工程技术-材料科学：综合

CiteScore

16.00

自引率

6.30%

发文量

4978

审稿时长

1.8 months

期刊介绍： ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.