Genomic and Metabolomic Analyses of Nocardiopsis maritima YSL2 as the Mycorrhizosphere Bacterium of Suaeda maritima (L.) Dumort

IF 3.3 2区生物学 Q2 CHEMISTRY, MEDICINAL Journal of Natural Products Pub Date : 2024-04-04 DOI:10.1021/acs.jnatprod.3c00843

Jaeyoun Lee, Soohyun Um, Eun-Hee Kim and Seung Hyun Kim*,

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Abstract

Nine bacteria were isolated from the episphere of Suaeda maritima (L.) Dumort. Among them, the bacterial strain YSL2 displayed the highest antimicrobial activity on agar plates and exhibited significant novelty compared with other bacteria based on 16S rRNA analysis. Consequently, Nocardiopsis maritima YSL2^T was subjected to phenotypic characterization and whole-genome sequencing. Phylogenetic analysis revealed its close association with Nocardiopsis aegyptia SNG49^T. Furthermore, genomic analysis of strain YSL2^T revealed the presence of various gene clusters, indicating its potential for producing antimicrobial secondary metabolites. Upon cultivation on a large scale, maritiamides A and B (1 and 2) were isolated and characterized as cyclic hexapeptides based on nuclear magnetic resonance, ultraviolet, infrared, and mass spectrometric data. The absolute configurations of the amino acid residues in the maritiamides were determined through chiral derivatization, utilizing FDAA and GITC. Maritiamides 1 and 2 exhibited promising antibacterial activities against Staphylococcus epidermidis and weakly inhibited the growth of Escherichia coli and Pseudomonas fluorescens.

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作为 Suaeda maritima (L.) Dumort 菌根层细菌的 Nocardiopsis maritima YSL2 的基因组和代谢组分析

从 Suaeda maritima (L.) Dumort 的表皮中分离出九种细菌。其中，细菌菌株 YSL2 在琼脂平板上显示出最高的抗菌活性，并且根据 16S rRNA 分析，与其他细菌相比显示出显著的新颖性。因此，对 Nocardiopsis maritima YSL2T 进行了表型鉴定和全基因组测序。系统进化分析表明它与 Nocardiopsis aegyptia SNG49T 关系密切。此外，对菌株 YSL2T 的基因组分析表明，该菌株存在多种基因簇，表明其具有产生抗菌次生代谢物的潜力。在大规模培养后，分离出了马立替酰胺 A 和 B（1 和 2），并根据核磁共振、紫外线、红外线和质谱数据对其环状六肽进行了表征。通过使用 FDAA 和 GITC 进行手性衍生，确定了马立替酰胺中氨基酸残基的绝对构型。马立替酰胺 1 和 2 对表皮葡萄球菌具有良好的抗菌活性，对大肠杆菌和荧光假单胞菌的生长有微弱的抑制作用。

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来源期刊

Journal of Natural Products 生物-药学

CiteScore

9.10

自引率

5.90%

发文量

294

审稿时长

2.3 months

期刊介绍： The Journal of Natural Products invites and publishes papers that make substantial and scholarly contributions to the area of natural products research. Contributions may relate to the chemistry and/or biochemistry of naturally occurring compounds or the biology of living systems from which they are obtained. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin. When new compounds are reported, manuscripts describing their biological activity are much preferred. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin.