Probing Effect of 6 MeV Electron Beam Irradiation on Haemoglobin Protein Using Spectroscopic Techniques

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-04-02 DOI:10.1177/15593258241240233

Sarika Hinge, Sanjay Dhole, Arun Banpurkar, Gauri Kulkarni

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Abstract

In this work, we study the effect of 6 MeV electron beam irradiation on the physicochemical properties of lyophilized Human Haemoglobin A (HbA). Electron beams generated from Race Track Microtron accelerator with energy 6 MeV were used to irradiate HbA at fluences of 5 × 1014 e−/cm2 and 10 × 1014 e−/cm2. Pristine and electron beam irradiated HbA were characterized using UV-visible and Fourier transform infrared spectroscopy (FTIR) spectroscopy. The interfacial tension of the aqueous solutions of HbA are also analysed by pendant drop method. Absorbance intensity, % transmittance and interfacial tension decrease with fluence. The peak position of the Soret band (λsoret = 404 nm) remains unaffected by the fluences. FTIR spectroscopy confirms the changes in the secondary structure of the haemoglobin. In the amide band I, the percentage of α-helix reduced from 8% to 1%, and an increase in β-sheet (19% to 29%) and β helix (6.3% to 15%) is observed. Interfacial tension decreases from 46.0 mN/m and 44.0 mN/m with increase in irradiation dose. These finding provides realistic guideline for biological cells exposure to electron beam radiation doses.

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利用光谱技术探测 6 MeV 电子束辐照对血红蛋白的影响

在这项工作中，我们研究了 6 MeV 电子束辐照对冻干人血红蛋白 A（HbA）理化性质的影响。由 Race Track Microtron 加速器产生的能量为 6 MeV 的电子束以 5 × 1014 e-/cm2 和 10 × 1014 e-/cm2 的通量辐照 HbA。使用紫外-可见光谱和傅立叶变换红外光谱（FTIR）对原始 HbA 和电子束辐照过的 HbA 进行了表征。此外，还采用垂滴法分析了 HbA 水溶液的界面张力。吸光强度、透射率和界面张力随通量的增加而降低。索雷特带的峰值位置（λsoret = 404 nm）不受通量的影响。傅立叶变换红外光谱证实了血红蛋白二级结构的变化。在酰胺带 I 中，α-螺旋的百分比从 8%下降到 1%，β-片状结构（19% 到 29%）和 β-螺旋（6.3% 到 15%）有所增加。随着辐照剂量的增加，界面张力分别从 46.0 mN/m 和 44.0 mN/m 下降。这些发现为生物细胞暴露于电子束辐射剂量提供了现实指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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