Determination of the chromosomal position effects for plug-and-play application in the Myxococcus xanthus chassis cells

IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Synthetic and Systems Biotechnology Pub Date : 2024-04-10 DOI:10.1016/j.synbio.2024.04.007
Xin-jing Yue, Jia-rui Wang, Jun-ning Zhao, Zhuo Pan, Yue-zhong Li
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Abstract

The chromosomal position effect can significantly affect the transgene expression, which may provide an efficient strategy for the inauguration of alien genes in new hosts, but has been less explored rationally. The bacterium Myxococcus xanthus harbors a large circular high-GC genome, and the position effect in this chassis may result in a thousand-fold expression variation of alien natural products. In this study, we conducted transposon insertion at TA sites on the M. xanthus genome, and used enrichment and dilution indexes to respectively appraise high and low expression potentials of alien genes at insertion sites. The enrichment sites are characteristically distributed along the genome, and the dilution sites are overlapped well with the horizontal transfer genes. We experimentally demonstrated the enrichment sites as high expression integration sites (HEISs), and the dilution sites unsuitable for gene integration expression. This work highlights that HEISs are the plug-and-play sites for efficient expression of integrated genes.

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确定染色体位置效应,以便在黄肉球菌底盘细胞中即插即用
染色体位置效应可显著影响转基因的表达,这可能为外来基因在新宿主中的就位提供了一种有效策略,但这方面的理性探索还较少。黄曲霉菌(Myxococcus xanthus)拥有一个大型环状高GC基因组,该底盘中的位置效应可能导致外来天然产物的表达量发生千倍变化。在这项研究中,我们在黄曲霉菌基因组的 TA 位点进行了转座子插入,并利用富集指数和稀释指数分别评估了外来基因在插入位点的高表达潜力和低表达潜力。富集位点沿基因组呈特征性分布,稀释位点则与水平转移基因有很好的重叠。我们通过实验证明,富集位点是高表达整合位点(HEIS),而稀释位点不适合基因整合表达。这项工作凸显了高表达整合位点是高效表达整合基因的即插即用位点。
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来源期刊
Synthetic and Systems Biotechnology
Synthetic and Systems Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
6.90
自引率
12.50%
发文量
90
审稿时长
67 days
期刊介绍: Synthetic and Systems Biotechnology aims to promote the communication of original research in synthetic and systems biology, with strong emphasis on applications towards biotechnology. This journal is a quarterly peer-reviewed journal led by Editor-in-Chief Lixin Zhang. The journal publishes high-quality research; focusing on integrative approaches to enable the understanding and design of biological systems, and research to develop the application of systems and synthetic biology to natural systems. This journal will publish Articles, Short notes, Methods, Mini Reviews, Commentary and Conference reviews.
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