Senling Guan, Wenfeng Zhou, Yongtang Yue, Songhe Wang, Bo Chen and Haishen Yang*,
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引用次数: 0
Abstract
Nicotine is the chief addictive ingredient in cigarettes, cigars, and snuff, and has extensive applications in the agricultural and pharmaceutical industries. The synthesis of nicotine using free enzyme systems has been widely reported in literature; this approach chiefly utilizes the alkaloid myosmine and the enzymes imine reductase (IRED) as well as glucose dehydrogenase (GDH), and generates the intermediate (S)-nornicotine. Free enzymes are not reusable, thereby resulting in higher cost of production. The use of recyclable immobilized enzymes is an efficient approach for lowering the costs and improving the efficiency of production. In the current study, we present an efficient and environment-friendly approach utilizing immobilized enzymes for synthesizing (S)-nornicotine using batch and continuous flow reaction processes. A highly active coimmobilized enzyme system was successfully obtained by coimmobilizing IRED and GDH on the resin LXTE-706. The immobilized enzymes were amenable to repeated usage for at least 40 operation cycles in the batch mode of operation and yielded a product with a high chiral purity of >99.90%, effectively reducing the overall production cost. Furthermore, a space–time yield of 211.47 g/Lh was obtained using a continuous mode of operation, which is 289.7-fold higher than that obtained with batch mode.
期刊介绍:
The journal Organic Process Research & Development serves as a communication tool between industrial chemists and chemists working in universities and research institutes. As such, it reports original work from the broad field of industrial process chemistry but also presents academic results that are relevant, or potentially relevant, to industrial applications. Process chemistry is the science that enables the safe, environmentally benign and ultimately economical manufacturing of organic compounds that are required in larger amounts to help address the needs of society. Consequently, the Journal encompasses every aspect of organic chemistry, including all aspects of catalysis, synthetic methodology development and synthetic strategy exploration, but also includes aspects from analytical and solid-state chemistry and chemical engineering, such as work-up tools,process safety, or flow-chemistry. The goal of development and optimization of chemical reactions and processes is their transfer to a larger scale; original work describing such studies and the actual implementation on scale is highly relevant to the journal. However, studies on new developments from either industry, research institutes or academia that have not yet been demonstrated on scale, but where an industrial utility can be expected and where the study has addressed important prerequisites for a scale-up and has given confidence into the reliability and practicality of the chemistry, also serve the mission of OPR&D as a communication tool between the different contributors to the field.