Recent advances in minimal fluorescent probes for optical imaging

IF 6.9 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Current Opinion in Chemical Biology Pub Date : 2024-04-25 DOI:10.1016/j.cbpa.2024.102458
Fabio de Moliner , Ferran Nadal-Bufi , Marc Vendrell
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Abstract

Fluorescent probes have revolutionized biological imaging by enabling the real-time visualization of cellular processes under physiological conditions. However, their size and potential perturbative nature can pose challenges in retaining the integrity of biological functions. This manuscript highlights recent advancements in the development of small fluorescent probes for optical imaging studies. Single benzene-based fluorophores offer versatility with minimal disruption, exhibiting diverse properties like aggregation-induced emission and pH responsiveness. Fluorescent nucleobases enable precise labeling of nucleic acids without compromising function, offering high sensitivity and compatibility with biochemistry studies. Bright yet small fluorescent amino acids provide an interesting alternative to bulky fusion proteins, facilitating non-invasive imaging of cellular events with high precision. These miniaturized fluorophores promise enhanced capabilities for studying biological systems in a non-invasive manner, fostering further innovations in molecular imaging.

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用于光学成像的最小荧光探针的最新进展
荧光探针能够实时显示生理条件下的细胞过程,从而彻底改变了生物成像技术。然而,荧光探针的尺寸和潜在的扰动特性会给保持生物功能的完整性带来挑战。本手稿重点介绍了用于光学成像研究的小型荧光探针的最新进展。单个苯基荧光探针以最小的干扰提供了多功能性,表现出多种特性,如聚集诱导发射和 pH 响应性。荧光核碱基能够在不影响功能的情况下精确标记核酸,具有高灵敏度,并与生物化学研究兼容。明亮而小巧的荧光氨基酸为庞大的融合蛋白提供了一种有趣的替代品,有助于对细胞事件进行高精度的非侵入性成像。这些微型荧光团有望增强以非侵入方式研究生物系统的能力,促进分子成像的进一步创新。
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来源期刊
Current Opinion in Chemical Biology
Current Opinion in Chemical Biology 生物-生化与分子生物学
CiteScore
13.30
自引率
1.30%
发文量
113
审稿时长
74 days
期刊介绍: COCHBI (Current Opinion in Chemical Biology) is a systematic review journal designed to offer specialists a unique and educational platform. Its goal is to help professionals stay informed about the growing volume of information in the field of Chemical Biology through systematic reviews.
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