Transcriptomic and proteomic features of a mouse model of sperm DNA damage induced by benzo(a)pyrene

IF 3.3 4区医学 Q2 REPRODUCTIVE BIOLOGY Reproductive toxicology Pub Date : 2024-04-17 DOI:10.1016/j.reprotox.2024.108596

Chenming Zhang , Yunfeng Ma , Wenbang Liu , Sicheng Ma , Zhelin Chen , XiaoHui Hao , Zixue Sun , Zulong Wang

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Abstract

This study replicated a mouse model of sperm DNA damage induced by benzo(a)pyrene (BaP), and the transcriptomic and proteomic features of the model were examined to clarify the pathways related to BaP-induced damage to sperm DNA. Male mice in the BaP group were subjected to BaP at a dosage of 100 mg/kg/d or an equivalent quantity of saline solution in the control group for 60 days. Subsequently, the DNA fragmentation index (DFI) in sperm was assessed using a sperm chromatin structure assay (SCSA). RNA-seq and data-independent acquisition (DIA) were used to identify the mRNA and protein expression patterns in the testis. The sperm DFI significantly increased in the BaP group. Compared to the control group, the BaP group exhibited differential expression of 240 genes (referred to as DEGs) and 616 proteins (referred to as DEPs). These molecules included Aldh1a1, Cyb5r3, Fads1, Oxsm, Rcn3, and Prss45. Pathways in cancer, the PI3K-Akt signaling pathway, metabolic pathways, and the MAPK signaling pathway were the primary areas where these genes showed enrichment. BaP can damage the DNA of sperm and affect metabolism, the PI3K-Akt pathway, and pathways associated with cancer signaling.

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苯并（a）芘诱导精子 DNA 损伤小鼠模型的转录组和蛋白质组特征

本研究复制了苯并（a）芘（BaP）诱导精子DNA损伤的小鼠模型，并研究了该模型的转录组和蛋白质组特征，以阐明BaP诱导精子DNA损伤的相关途径。雄性小鼠在BaP组中以100毫克/千克/天的剂量摄入BaP，或在对照组中摄入等量的生理盐水，持续60天。随后，使用精子染色质结构检测法（SCSA）评估精子中的 DNA 断裂指数（DFI）。RNA-seq和数据独立采集（DIA）被用来确定睾丸中mRNA和蛋白质的表达模式。BaP组的精子DFI明显增加。与对照组相比，BaP 组表现出 240 个基因（称为 DEGs）和 616 个蛋白质（称为 DEPs）的差异表达。这些分子包括 Aldh1a1、Cyb5r3、Fads1、Oxsm、Rcn3 和 Prss45。癌症通路、PI3K-Akt 信号通路、代谢通路和 MAPK 信号通路是这些基因出现富集的主要领域。BaP会损伤精子的DNA，影响新陈代谢、PI3K-Akt通路以及与癌症信号转导相关的通路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproductive toxicology 生物-毒理学

CiteScore

6.50

自引率

3.00%

发文量

131

审稿时长

45 days

期刊介绍： Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.