Identification of Codonopsis Radix from different sources by integrating high‐performance liquid chromatography fingerprinting with chemometric approaches and Lobetyolin determination

IF 17.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-04-24 DOI:10.1002/sscp.202400027

Xuxia Liu, Xiaoling Liu, Xin Wang, Zhengjun Chen, Danhui Nan, Wenrong Luo, Fude Yang

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Abstract

Codonopsis Radix (CR) is often used as both a medicine and a food ingredient. However, identifying CR can be difficult due to various factors such as variety, sulfur fumigation, and storage time. Consequently, a comprehensive strategy based on fingerprinting and chemometric approaches was developed to distinguish different CRs. First, the fingerprints of CR were acquired by high‐performance liquid chromatography (HPLC). Secondly, various chemical pattern recognition methods were employed to identify CR. Additionally, 14 and 16 major compounds were identified as marker compounds to differentiate between different varieties and sulfur‐fumigated samples, respectively. Furthermore, the amount of Lobetyolin present in different samples was determined, showing that the content of Lobetyolin varied significantly across the samples. Codonopsis pilosula Nannf. var. modesta (Nannf.) L.T.Shen had the highest content of Lobetyolin, followed by Codonopsis tangshen Oliv., and Codonopsis pilosula (Franch.) Nannf. In addition, the content of Lobetyolin was highest when it was non‐sulfur‐fumigated and stored for 0 years. Conclusively, HPLC fingerprint in conjunction with chemical pattern recognition and component content determination, can be employed to differentiate different varieties and sulfur‐fumigated CR. Additionally, it is a reliable, comprehensive, simple, and rapid method for the identification of CR.

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通过将高效液相色谱指纹图谱与化学计量学方法和洛贝托林测定相结合，鉴定不同来源的党参

党参 (CR) 经常被用作药物和食品配料。然而，由于品种、硫磺熏蒸和储存时间等各种因素的影响，鉴别党参可能比较困难。因此，我们开发了一种基于指纹图谱和化学计量学方法的综合策略来区分不同的 CR。首先，通过高效液相色谱法（HPLC）获得了 CR 的指纹图谱。其次，采用各种化学模式识别方法来鉴定 CR。此外，还确定了 14 种和 16 种主要化合物作为标记化合物，分别用于区分不同品种和硫磺熏蒸过的样品。此外，还测定了不同样品中 Lobetyolin 的含量，结果表明不同样品中 Lobetyolin 的含量差异很大。L.T.Shen 的 Lobetyolin 含量最高，其次是 Codonopsis tangshen Oliv.和 Codonopsis pilosula (Franch.) Nannf.此外，未经硫磺熏蒸且贮藏 0 年的党参中 Lobetyolin 的含量最高。最后，HPLC指纹图谱与化学模式识别和成分含量测定相结合，可用于区分不同品种和硫磺熏蒸过的 CR。此外，它还是一种可靠、全面、简单和快速的 CR 鉴定方法。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.