Darius Hoven, Misaki Inaoka, Reece McCoy, A. Withers, Róisín M. Owens, G. Malliaras
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引用次数: 0
Abstract
Microelectrode arrays (MEAs) have applications in drug discovery, toxicology, and basic research. They measure the electrophysiological response of tissue cultures to quantify changes upon exposure to biochemical stimuli. Unfortunately, manual addition of chemicals introduces significant noise in the recordings. Here, we report a simple-to-fabricate fluidic system that addresses this issue. We show that cell cultures can be successfully established in the fluidic compartment under continuous flow conditions and that the addition of chemicals introduces minimal noise in the recordings. This dynamic cell culture system represents an improvement over traditional tissue culture wells used in MEAs, facilitating electrophysiology measurements.
Graphical abstract
微电极阵列（MEA）可应用于药物发现、毒理学和基础研究。它们测量组织培养物的电生理反应，量化暴露于生化刺激时的变化。遗憾的是，人工添加化学物质会在记录中引入大量噪声。在这里，我们报告了一种易于制造的流体系统，它可以解决这个问题。我们的研究表明，细胞培养可以在连续流动条件下成功地在流体室中建立，而且添加化学物质在记录中带来的噪音极小。这种动态细胞培养系统是对 MEA 中使用的传统组织培养井的一种改进，有助于电生理学测量。图表摘要
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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