How Inert is Single-Stranded DNA Towards DNA-Stabilized Silver Nanoclusters? A Case Study

IF 3 4区化学 Q3 CHEMISTRY, PHYSICAL ChemPhotoChem Pub Date : 2024-04-22 DOI:10.1002/cptc.202400014

Vanessa Rück, Cecilia Cerretani, Tom Vosch

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Abstract

A case study, detailing the effect of different DNA oligomers on a NIR-emitting DNA-stabilized silver nanocluster (DNA-AgNC), is reported. The presence of single-stranded DNA oligomers was found to adversely affect the chemical stability of (DNA)₂[Ag₁₆Cl₂]⁸⁺ with distinct degrees of destruction depending on the DNA sequence. To increase the chemical stability of the DNA-AgNC, we implemented two protection strategies. First, hybridization of the bare DNA strands with the corresponding complementary sequences dramatically reduced the destruction of (DNA)₂[Ag₁₆Cl₂]⁸⁺, as demonstrated by the decreased drops in both the absorption and emission spectra. Secondly, saturation of the free DNA oligomers with silver cations left (DNA)₂[Ag₁₆Cl₂]⁸⁺ intact. Our investigation can thus provide an easy-to-implement approach to discover DNA sequences that are intrinsically less reactive towards preformed DNA-AgNCs, and give an idea on how to protect DNA-AgNCs from bare DNA strands.

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单链 DNA 对 DNA 稳定的纳米银团簇的惰性如何？案例研究。

本文报告了一项案例研究，详细阐述了不同 DNA 链对近红外发光 DNA 稳定银纳米簇（DNA-AgNC）的影响。研究发现，单链 DNA 寡聚体的存在会对 (DNA)2[Ag16Cl2]8+ 的化学稳定性产生不利影响，根据 DNA 序列的不同，破坏程度也不同。为了提高 DNA-AgNC 的化学稳定性，我们采用了两种保护策略。首先，将裸 DNA 链与相应的互补序列杂交，大大降低了 (DNA)2[Ag16Cl2]8+ 的破坏程度，吸收光谱和发射光谱中的降幅都有所减小就证明了这一点。其次，用银阳离子钝化裸 DNA 寡聚体后，(DNA)2[Ag16Cl2]8+ 依然完好无损。因此，我们的研究提供了一种简便易行的方法来发现对预制 DNA-AgNCs 反应性较低的 DNA 序列，并为如何保护 DNA-AgNCs 免受裸 DNA 链的影响提供了思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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