First report of amphotericin B resistant Candida haemulonii isolated from the ICU of a referral hospital in Indonesia

IF 17.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-04-15 DOI:10.18502/ijm.v16i2.15363

Rifdah Hanifah, Mardiastuti Wahid, Andi Yasmon

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Abstract

Background and Objectives: Amphotericin B is a broad-spectrum antifungal agent commonly used to treat Candida haemulonii infection. C. haemulonii was isolated from patients reported to be intrinsically resistant to amphotericin B, en- coded by the ERG2 and ERG11 genes. However, there have been limited studies concerning amphotericin B-resistant C. haemulonii in Indonesia. The objective of this study is to explore the phenotypic and genotypic characteristics (ERG2 and ERG11) of C. haemulonii isolated from the ICU of a referral hospital in Indonesia. Materials and Methods: Identification and susceptibility tests were conducted using VITEK2. Thereafter, DNA was ex-tracted and amplified using conventional PCR followed by DNA sequencing (Sanger method). Results: The results of the phenotypic susceptibility test showed that all C. haemulonii were resistant to amphotericin B. ERG2 and ERG11 sequences showed the same amino acid sequence and corresponded to references that are resistant to amphotericin B. Conclusion: The resistant properties of C. haemulonii against amphotericin B found in this study require further exploration, including comparing resistant and sensitive C. haemulonii to amphotericin B. In addition, it is necessary to analyze other genes besides ERG2 and ERG11.

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首次报告从印度尼西亚一家转诊医院的重症监护室分离出对两性霉素 B 耐药的血念珠菌

背景和目的：两性霉素 B 是一种广谱抗真菌药物，常用于治疗血念珠菌感染。据报道，从患者体内分离出的血念珠菌对ERG2和ERG11基因编码的两性霉素B具有内在耐药性。然而，有关印度尼西亚耐两性霉素 B 的 C. haemulonii 的研究还很有限。本研究旨在探讨从印度尼西亚一家转诊医院重症监护室分离出的血吸虫的表型和基因型特征（ERG2和ERG11）。材料与方法：使用 VITEK2 进行鉴定和药敏试验，然后使用常规 PCR 提取和扩增 DNA，再进行 DNA 测序（桑格方法）。结果ERG2和ERG11序列显示了相同的氨基酸序列，并与对两性霉素B有抗药性的参照物相对应：本研究中发现的血球孢子菌对两性霉素 B 的耐药性需要进一步探讨，包括比较血球孢子菌对两性霉素 B 的耐药性和敏感性。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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