Cytotoxic activity and apoptosis induction by supernatant of Lentilactobacillus buchneri on HT-29 colon cancer cells

IF 17.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-04-15 DOI:10.18502/ijm.v16i2.15355

Adel Abedi, F. Tafvizi, Neda Akbari, Parvaneh Jafari

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Abstract

Background and Objectives: Colorectal cancer (CRC) is the fourth most commonly diagnosed cancer and the third most deadly cancer in the world. According to recent experimental reports, probiotics and their derivatives protect CRC patients from treatment-related side effects. Therefore, the present study aimed to investigate the cytotoxic impact of the cell-free supernatant (CFS) of Lentilactobacillus buchneri on the HT-29 cancer cell line. Materials and Methods: In the current study, we used the L. buchneri CFS, which was well isolated and identified in our previous investigation from traditional yogurt in the Arak region of Iran. The apoptosis induction in HT-29 cancer cells was assessed by cell cytotoxicity, flow cytometry, and qRT-PCR. Results: L. buchneri CFS inhibited the proliferation of HT-29 cancer cells in a time- and dose-dependent manner. The apoptotic effect of CFS was further supported by the flow cytometry data, which showed that the maximum incidence of apoptosis 50 was observed in HT-29 cancer cells treated with the IC concentration of CFS after 72 hours. CFS of L. buchneri also exerted the up-regulating effect on the expression of pro-apoptotic genes including BAX, CASP9, and CASP3. L. buchneri CFS at an IC 50 dose induced cell cycle arrest in the G0/G1 phase in HT-29 cells. Conclusion: This study indicates that L. buchneri CFS can prevent colorectal cancer (CRC) development in patients by inducing cancer cell apoptosis. This finding suggests that the CFS of L. buchneri could be used as a therapeutic agent for the control of CRC.

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布氏扁豆乳杆菌上清液对 HT-29 结肠癌细胞的细胞毒活性和凋亡诱导作用

背景和目标：结肠直肠癌（CRC）是全球第四大常见癌症，也是第三大致命癌症。根据最近的实验报告，益生菌及其衍生物能保护 CRC 患者免受治疗相关副作用的影响。因此，本研究旨在探讨布氏扁豆乳杆菌无细胞上清液（CFS）对 HT-29 癌细胞系的细胞毒性影响。材料与方法：在本研究中，我们使用了布氏扁豆乳杆菌无细胞上清液（CFS），该上清液在之前的调查中已从伊朗阿拉克地区的传统酸奶中很好地分离和鉴定出来。通过细胞毒性、流式细胞仪和 qRT-PCR 对 HT-29 癌细胞的凋亡诱导进行了评估。结果显示布氏乳酸菌 CFS 以时间和剂量依赖性的方式抑制 HT-29 癌细胞的增殖。流式细胞术数据进一步证实了 CFS 的凋亡效应，该数据显示，用 IC 浓度的 CFS 处理 HT-29 癌细胞 72 小时后，观察到最大凋亡发生率为 50。布氏酵母菌 CFS 还能上调促凋亡基因（包括 BAX、CASP9 和 CASP3）的表达。IC 50剂量的布氏酵母菌CFS可诱导HT-29细胞的细胞周期停滞在G0/G1期。结论本研究表明，布氏酵母菌 CFS 可通过诱导癌细胞凋亡来预防患者患上结直肠癌（CRC）。这一发现表明，布氏酵母菌 CFS 可用作控制 CRC 的治疗药物。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.