Immunomodulatory-associated gene transcripts to multipotency of bovine amniotic fluid mesenchymal stem cells

IF 17.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-04-12 DOI:10.1590/1984-3143-AR2023-0155

J. C. Baptistella, C. G. da Silva, Sônia Nair Báo, Letícia Colin Panegossi, Tereza Cristina Cardoso, Roberto Gameiro de Carvalho, C. F. Martins

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Abstract

Abstract The adnexa fetal tissues are sources of mesenchymal stromal cells (MSCs) due to their noninvasive harvest, with all biological material discarded most of the time. MSCs are a promise regarding to their plasticity, self-renewal, differentiation potentials, immunomodulatory and anti-inflammatory properties, which have made clinical stem cell therapy a reality. The present study aimed to characterize and evaluate the immunomodulation ability of bovine mesenchymal cells collected from bovine amniotic fluid (bAFMSCs) isolated and subjected to sixth consecutive culture passages in vitro. The multilineage properties of the bAFMSCs collections confirmed the ability to undergo adipogenic, chondrogenic and osteogenic differentiation. The mesenchymal gene transcription CD106, CD73, CD29, CD90 and CD166 were detected in bAFMSCs, whereas CD34 and CD45 were not detected. Regarding cytokine mRNA expression, IL2, IL6, INFα, INFβ, INFγ, TNFα and TNFβ were downregulated, while IL10 was highly regulated in all studied passages. The present study demonstrated the immunological properties and multipotency of in vitro bAFMSCs collections, and thus, they can be tested in cattle pathological treatments or multiplication by nuclear transfer cloning.

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与牛羊水间充质干细胞多潜能性相关的免疫调节基因转录本

摘要胎儿的附件组织是间充质基质细胞（MSCs）的来源，因为它们是无创采集的，而所有生物材料在大多数情况下都会被丢弃。间充质干细胞具有可塑性、自我更新能力、分化潜能、免疫调节和抗炎特性，这使临床干细胞治疗成为现实。本研究旨在表征和评估从牛羊水中收集的牛间充质细胞（bAFMSCs）的免疫调节能力。所收集的牛间充质干细胞的多线特性证实了其具有成脂、成软骨和成骨分化能力。在 bAFMSCs 中检测到了间充质基因转录 CD106、CD73、CD29、CD90 和 CD166，而 CD34 和 CD45 则未检测到。在细胞因子 mRNA 表达方面，IL2、IL6、INFα、INFβ、INFγ、TNFα 和 TNFβ 均出现下调，而 IL10 在所有研究的细胞中均受到高度调控。本研究证明了体外 bAFMSCs 的免疫学特性和多潜能性，因此可用于牛的病理治疗或通过核移植克隆进行繁殖。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.