Resistome analysis of Escherichia coli isolates from layers in Hungary.

IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES Acta veterinaria Hungarica Pub Date : 2024-04-05 DOI:10.1556/004.2024.00988
Á. Kerek, Ábel Szabó, Krisztián Bányai, Eszter Kaszab, Krisztina Bali, M. Papp, László Kovács, Ákos Jerzsele
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Abstract

The authors aimed to investigate eight strains of Escherichia coli (E. coli) strains from Hungarian layer flocks for antimicrobial resistance genes (ARG), using metagenomic methods. The strains were isolated from cloacal swabs of healthy adult layers. This study employed shotgun sequencing-based genetic and bioinformatic analysis along with determining phenotypic minimum inhibitory concentrations. A total of 59 ARGs were identified in the eight E. coli isolates, carrying ARGs against 15 groups of antibiotics. Among these, 28 ARGs were identified as transferable. Specifically, four ARGs were plasmid-derived, 18 ARGs were phage-derived and an additional six ARGs were predicted to be mobile, contributing to their mobility and potential spread between bacteria.
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对从匈牙利地层中分离出的大肠埃希菌进行抗性组分析。
作者采用元基因组学方法对匈牙利蛋鸡群中的八株大肠埃希氏菌(E. coli)进行了抗菌药耐药性基因(ARG)研究。这些菌株是从健康成年蛋鸡的泄殖腔拭子中分离出来的。这项研究采用了基于枪式测序的遗传和生物信息分析方法,并确定了表型最小抑菌浓度。在 8 个大肠杆菌分离物中共鉴定出 59 个 ARGs,携带针对 15 组抗生素的 ARGs。其中,28 个 ARGs 被鉴定为可转移的。具体来说,4 个 ARGs 来自质粒,18 个 ARGs 来自噬菌体,另有 6 个 ARGs 被预测为可移动的,这有助于它们的移动性和在细菌间的潜在传播。
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来源期刊
Acta veterinaria Hungarica
Acta veterinaria Hungarica 农林科学-兽医学
CiteScore
1.80
自引率
0.00%
发文量
39
审稿时长
>36 weeks
期刊介绍: Acta Veterinaria Hungarica publishes original research papers presenting new scientific results of international interest, and to a limited extent also review articles and clinical case reports, on veterinary physiology (physiological chemistry and metabolism), veterinary microbiology (bacteriology, virology, immunology, molecular biology), on the infectious diseases of domestic animals, on veterinary parasitology, pathology, clinical veterinary science and reproduction.
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