{"title":"Advantages and Limitations of the Biofilm Study Methods","authors":"D. H. Mir, M. A. Rather","doi":"10.1134/S000368382402011X","DOIUrl":null,"url":null,"abstract":"<p>A community of microorganisms adhered to a solid surface and entrapped in a self-produced extracellular polymeric matrix, in general is referred to as a ‘biofilm’. Biofilm formation is a phenomenon naturally exhibited by the majority of microorganisms, (except a few like <i>Pelagibacter</i> and some planktonic bacteria). The trait of biofilm formation by the microorganisms allows them to survive in harsh environments, important for their existence and sustenance. To form biofilms, microorganisms possess a supportive genetic makeup, which enables them to function and grow in a coordinated manner. Surprisingly at present, there is a lack of proper consensus regarding the utilization of technique and methodologies available in the precise analysis, growth and characterization of biofilms. The current review aims to bridge this gap by presenting a detailed review of the available study methods and tools the most promising in the detection, growth and characterization of biofilms. The selection of the most appropriate biofilm study and analysis method in a given situation is of utmost importance in order to understand the formation and prospective fruitful utilization of biofilms by humankind.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 2","pages":"264 - 279"},"PeriodicalIF":1.0000,"publicationDate":"2024-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S000368382402011X","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
A community of microorganisms adhered to a solid surface and entrapped in a self-produced extracellular polymeric matrix, in general is referred to as a ‘biofilm’. Biofilm formation is a phenomenon naturally exhibited by the majority of microorganisms, (except a few like Pelagibacter and some planktonic bacteria). The trait of biofilm formation by the microorganisms allows them to survive in harsh environments, important for their existence and sustenance. To form biofilms, microorganisms possess a supportive genetic makeup, which enables them to function and grow in a coordinated manner. Surprisingly at present, there is a lack of proper consensus regarding the utilization of technique and methodologies available in the precise analysis, growth and characterization of biofilms. The current review aims to bridge this gap by presenting a detailed review of the available study methods and tools the most promising in the detection, growth and characterization of biofilms. The selection of the most appropriate biofilm study and analysis method in a given situation is of utmost importance in order to understand the formation and prospective fruitful utilization of biofilms by humankind.
期刊介绍:
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.