Can Mitotic Figures in Hyperchromatic Crowded Groups be Cytodiagnostic Criteria for High-Grade Squamous Intra-epithelial Lesions?

IF 17.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-04-01 DOI:10.4103/joc.joc_156_23

H. Suzuki, Yumeno Kondo, Chihiro Oda, Takeshi Nishikawa, Mao Takeuchi, Shigenobu Tatsumi, Sho Hosokawa, S. Irino, Tomoko Uchiyama, Tomomi Fujii, Yoshiaki Norimatsu

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Abstract

The present study aimed to investigate whether the presence of mitoses in hyperchromatic crowded groups (HCGs) in cervical cytological specimens can serve as cytological criteria for high-grade squamous intra-epithelial lesions (HSILs). Various parameters were examined, including the frequency of mitotic figures per high power field (HPF) in Pap, hematoxylin eosin (HE) samples, and PHH3 immunocytochemical (ICC) and immunohistochemical (IHC) analyses. In the Pap and PHH3-ICC samples, the number of mitotic figures observed in HCGs was significantly higher in HSIL (P < 0.001) compared to other groups. Furthermore, the frequency of observing two or more mitoses was significantly higher in HSIL (Pap: P = 0.002, PHH3-ICC: P < 0.001) than in low-grade squamous intra-epithelial lesions (LSILs). Moreover, a comparison between Pap samples and PHH3-ICC showed that the frequency of two or more mitoses was significantly higher in the PHH3-ICC analysis of HSIL (P = 0.042). Regarding HE and PHH3-IHC samples, counting the number of mitoses in the lower and middle/upper layers of the squamous epithelial layer revealed that HSIL had a significantly higher value (HE: P = 0.0089, PHH3-IHC: P = 0.0002) than LSIL in the middle/upper layers. Hence, the presence of two or more mitotic figures in HCGs per HPF in cervical cytology indicates a suspicion of HSIL. The detection of mitoses in PHH3-ICC samples is more sensitive and easier to observe than in Pap samples, making it a valuable mitotic marker.

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高色素拥挤群中的有丝分裂图能否作为高级别鳞状上皮内病变的细胞诊断标准？

本研究旨在探讨宫颈细胞学标本中高色素人群（HCGs）有丝分裂是否可作为高级别鳞状上皮内病变（HSILs）的细胞学标准。我们对各种参数进行了检测，包括巴氏涂片、苏木精伊红（HE）样本中每个高倍视野（HPF）的有丝分裂数频率，以及 PHH3 免疫细胞化学（ICC）和免疫组织化学（IHC）分析。在巴氏涂片和 PHH3-ICC 样本中，HCG 观察到的有丝分裂数目在 HSIL 中明显高于其他组别（P < 0.001）。此外，HSIL（巴氏试验：P = 0.002，PHH3-ICC：P < 0.001）中观察到两个或两个以上有丝分裂的频率明显高于低级别鳞状上皮内病变（LSIL）。此外，巴氏涂片样本与 PHH3-ICC 的比较显示，在对 HSIL 的 PHH3-ICC 分析中，两个或两个以上有丝分裂的频率明显更高（P = 0.042）。在 HE 和 PHH3-IHC 样本中，计算鳞状上皮下层和中/上层的有丝分裂数发现，在中/上层，HSIL 的数值明显高于 LSIL（HE：P = 0.0089，PHH3-IHC：P = 0.0002）。因此，在宫颈细胞学检查中，每 HPF 的 HCG 中出现两个或两个以上的有丝分裂像就表明怀疑有 HSIL。在 PHH3-ICC 样本中检测有丝分裂比在巴氏涂片样本中检测有丝分裂更敏感、更容易观察，因此是一种有价值的有丝分裂标记物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.