Influence of benzothiadiazole on isoprene metabolism and aroma accumulation in Chardonnay grapes (Vitis vinifera L.) during development

IF 3 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY European Food Research and Technology Pub Date : 2024-04-01 DOI:10.1007/s00217-024-04503-1

Yuqi Han, Jianfeng Wang, Chunxia Chen, Ruwen Guan, Yu Zhang, Yumei Jiang

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Abstract

Chardonnay is a non-floral grape with economic value and nutritional properties that is widely grown in northwestern China. However, limited research has been conducted on exogenous spraying to regulate volatile compound metabolism (especially isoprene metabolism) in Chardonnay grapes. Benzothiadiazole (BTH) can induce disease resistance and augment the contents of phenolics and volatile compounds in grapes. This study aimed to evaluate the influence of BTH on the accumulation of isoprene aroma, carotenoid levels and the activity profiles of key enzymes. Chardonnay grapevines were treated with 0.37 mM BTH at veraison to investigate the impact on physicochemical parameters, carotenoids, metabolic enzyme activities, and free and bound isoprenoids in developing Chardonnay grapes. The results showed that reducing sugars, and total soluble solids of BTH-treated grapes increased, while the content of titratable acidity, β-carotene, zeaxanthin, β-cryptoxanthin, chlorophyll, and total carotenoids in treated ripe grapes decreased relative to control berries. BTH-treated ripe grapes exhibited elevated levels of bound norisoprenoids and three key enzemy activities (carotenoid cleavage dioxygenase, phytoene synthase, lycopene β-cyclase), with a slight decrease in free norisoprenoids. Free and bound terpenoids profiles were also significantly enhanced by BTH treatment at veraison. Our findings offer novel perspectives into the modulation of isoprene biosynthesis in grape berries when exposed to BTH.

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苯并噻二唑对霞多丽葡萄（Vitis vinifera L.）生长过程中异戊二烯代谢和香气积累的影响

霞多丽是一种具有经济价值和营养价值的非花葡萄，在中国西北地区广泛种植。然而，关于外源喷施调节霞多丽葡萄挥发性化合物代谢（尤其是异戊二烯代谢）的研究还很有限。苯并噻二唑（BTH）可诱导葡萄抗病，提高酚类化合物和挥发性化合物的含量。本研究旨在评估 BTH 对异戊二烯香气积累、类胡萝卜素水平和关键酶活性的影响。在葡萄成熟期用 0.37 mM BTH 处理霞多丽葡萄藤，研究其对理化参数、类胡萝卜素、代谢酶活性以及游离和结合异戊二烯的影响。结果表明，BTH 处理的葡萄还原糖和总可溶性固形物含量增加，而可滴定酸度、β-胡萝卜素、玉米黄质、β-隐黄质、叶绿素和总类胡萝卜素的含量相对于对照果实有所下降。经 BTH 处理的成熟葡萄中，结合的norisoprenoids 和三种关键酶活性（类胡萝卜素裂解二氧酶、植物烯合成酶、番茄红素 β-环化酶）水平升高，游离的norisoprenoids 水平略有下降。游离萜类化合物和结合萜类化合物的含量也因 BTH 处理而在成熟期显著增加。我们的研究结果为研究暴露于 BTH 时葡萄浆果中异戊二烯的生物合成调控提供了新的视角。

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来源期刊

European Food Research and Technology 工程技术-食品科技

CiteScore

6.60

自引率

3.00%

发文量

232

审稿时长

2.0 months

期刊介绍： The journal European Food Research and Technology publishes state-of-the-art research papers and review articles on fundamental and applied food research. The journal''s mission is the fast publication of high quality papers on front-line research, newest techniques and on developing trends in the following sections: -chemistry and biochemistry- technology and molecular biotechnology- nutritional chemistry and toxicology- analytical and sensory methodologies- food physics. Out of the scope of the journal are: - contributions which are not of international interest or do not have a substantial impact on food sciences, - submissions which comprise merely data collections, based on the use of routine analytical or bacteriological methods, - contributions reporting biological or functional effects without profound chemical and/or physical structure characterization of the compound(s) under research.