Inhibitory Effect of Ginkgo biloba Extract on Oxidative Stress and Apoptosis of Myocardial Cells in Sepsis by Regulating miR-370/FOXO1

Abstract

This research was aimed to investigate the effects of Ginkgo biloba leaf extract on oxidative stress and apoptosis in septic cardiomyocytes, focusing on the role of the miR-370/FOXO1 regulatory pathway. The major components of Ginkgo biloba leaf extract were determined using high-performance liquid chromatography. In vitro cardiomyocytes were utilized as the research subjects, with control (Con) group cardiomyocytes obtained from normal individuals, experimental (Exp) group cardiomyocytes obtained from patients with septic cardiomyocyte injury, and treatment (Tre) group cardiomyocytes obtained from septic cardiomyocyte injury patients treated with Ginkgo biloba leaf extract. Western blot and real-time fluorescence quantification techniques were employed to investigate cell apoptosis, changes in antioxidative enzymes superoxide dismutase (SOD) and malondialdehyde (MDA) levels, as well as alterations in key proteins involved in oxidative stress and the miR-370/FOXO1 regulatory pathway. The results showed that the main components of Ginkgo biloba leaf extract included total flavonoids and total Ginkgo biloba acid. Treatment with Ginkgo biloba leaf extract markedly reduced oxidative stress levels and exhibited a notable decrease in apoptosis rate. The SOD concentration in Exp group cells was greatly decreased (P <0.05), and the SOD concentration in Tre group cells was drastically inferior to that in Exp group (P <0.05). The MDA concentration in Exp group cells was notably increased (P <0.05), while the MDA concentration in Tre group cells was drastically inferior to that in Exp group (P <0.05). The levels of Caspase-3, Caspase-6, Bax/BCL-2, and FOXO1 proteins were markedly elevated in Exp group cardiomyocytes (P <0.05), while miR-370 was greatly reduced (P <0.05). In Tre group cardiomyocytes, the levels of Caspase-3, Caspase-6, Bax/BCL-2, and FOXO1 proteins were drastically inferior to those in Exp group (P <0.05), and miR-370 was notably superior to Exp group (P < 0.05). In summary, Ginkgo biloba leaf extract can inhibit oxidative stress and apoptosis in septic cardiomyocytes by modulating the miR-370/FOXO1 pathway, providing a novel approach for the treatment of septic cardiomyocyte injury.