The Effect of Bacillus subtilis in Combination with Chitosan Salicylate on Peroxidase and Catalase Activity in B. sorokiniana Infected Wheat

IF 1 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied Biochemistry and Microbiology Pub Date : 2024-04-21 DOI:10.1134/S0003683824020121
I. I. Novikova, E. V. Popova, N. M. Kovalenko, I. L. Krasnobaeva
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Abstract

The aim of this work was to study the effect of Bacillus subtilis strains VKM B-2604D and VKM B-2605D, which form the basis of the Vitaplan biological product, and their combinations with chitosan salicylate on antioxidant defense enzymes in the process of formation of resistance of wheat plants to infection with the dark brown blotch pathogen Bipolaris sorokiniana. In wheat plants treated with B. subtilis strains and their combinations with chitosan salicylate, upon subsequent infection, catalase and peroxidase are activated, which regulate the intensity of oxidative stress induced by the introduction of the pathogen. Taking the data we obtained into account, it can be assumed that the increase in plant resistance to the pathogen is realized through the control of the activity of antioxidant enzymes (in particular, catalase and peroxidase), which maintain the concentration of H2O2 at the level necessary for the neutralization of the phytopathogen in tissues, including the direct destruction of the invading pathogen and/or activation of crosslinking and lignification of the cell wall. These processes strengthen the cell wall and help contain the spread of the pathogen in the plant. At the same time, protective reactions are also switched on, leading to the development of induced resistance in wheat plants to dark brown spotting, which manifests itself in a decrease in the development of the disease by 25–45% relative to the infected control in the treated plants. The results indicate that the combination of active strains of microorganisms-antagonists of plant pathogens and chitosan salicylate is promising for increasing the biological efficiency and expanding the spectrum of action of the developed biological products.

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枯草芽孢杆菌与壳聚糖水杨酸盐复配对受 B. sorokiniana 感染小麦过氧化物酶和过氧化氢酶活性的影响
这项工作的目的是研究构成维他普兰生物产品基础的枯草芽孢杆菌菌株 VKM B-2604D 和 VKM B-2605D 以及它们与壳聚糖水杨酸盐的组合在小麦植株形成对深褐色斑点病原体 Bipolaris sorokiniana 感染的抗性过程中对抗氧化防御酶的影响。在用枯草杆菌菌株及其与壳聚糖水杨酸盐的组合处理过的小麦植株中,在随后的感染过程中,过氧化氢酶和过氧化物酶被激活,从而调节病原体引入所诱发的氧化应激的强度。根据我们获得的数据,可以推测植物对病原体的抗性是通过控制抗氧化酶(特别是过氧化氢酶和过氧化物酶)的活性实现的,这些酶将 H2O2 的浓度维持在中和组织中植物病原体所需的水平,包括直接破坏入侵的病原体和/或激活细胞壁的交联和木质化。这些过程可强化细胞壁,有助于遏制病原体在植物体内的扩散。与此同时,保护性反应也被开启,导致小麦植株对暗褐斑病产生诱导抗性,表现为与受感染的对照组相比,处理过的植株发病率降低 25-45%。研究结果表明,将植物病原体拮抗剂微生物活性菌株与壳聚糖水杨酸盐相结合,有望提高生物效率,扩大所开发生物产品的作用范围。
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来源期刊
Applied Biochemistry and Microbiology
Applied Biochemistry and Microbiology 生物-生物工程与应用微生物
CiteScore
1.70
自引率
12.50%
发文量
75
审稿时长
6-12 weeks
期刊介绍: Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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