I. I. Novikova, E. V. Popova, N. M. Kovalenko, I. L. Krasnobaeva
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引用次数: 0
Abstract
The aim of this work was to study the effect of Bacillus subtilis strains VKM B-2604D and VKM B-2605D, which form the basis of the Vitaplan biological product, and their combinations with chitosan salicylate on antioxidant defense enzymes in the process of formation of resistance of wheat plants to infection with the dark brown blotch pathogen Bipolaris sorokiniana. In wheat plants treated with B. subtilis strains and their combinations with chitosan salicylate, upon subsequent infection, catalase and peroxidase are activated, which regulate the intensity of oxidative stress induced by the introduction of the pathogen. Taking the data we obtained into account, it can be assumed that the increase in plant resistance to the pathogen is realized through the control of the activity of antioxidant enzymes (in particular, catalase and peroxidase), which maintain the concentration of H2O2 at the level necessary for the neutralization of the phytopathogen in tissues, including the direct destruction of the invading pathogen and/or activation of crosslinking and lignification of the cell wall. These processes strengthen the cell wall and help contain the spread of the pathogen in the plant. At the same time, protective reactions are also switched on, leading to the development of induced resistance in wheat plants to dark brown spotting, which manifests itself in a decrease in the development of the disease by 25–45% relative to the infected control in the treated plants. The results indicate that the combination of active strains of microorganisms-antagonists of plant pathogens and chitosan salicylate is promising for increasing the biological efficiency and expanding the spectrum of action of the developed biological products.
期刊介绍:
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.