{"title":"Identificación y actividad antifúngica de bacterias halófilas aisladas de suelos salinos en Campeche, México","authors":"","doi":"10.1016/j.ram.2024.02.004","DOIUrl":null,"url":null,"abstract":"<div><p>Phytopathogenic fungi <em>Alternaria alternata</em> and <em>Colletotrichum gloeosporioide</em>s cause diseases in plant tissues as well as significant postharvest losses. The use of chemical fungicides for their control has negative effects on health and the environment. Secondary metabolites from halophilic bacteria are a promising alternative for new antifungal compounds. In the present study, halophilic bacteria were isolated and characterized from two sites with saline soils called branquizales in Campeche, Mexico. A total of 64 bacteria were isolated. <em>Agrobacterium</em>, <em>Bacillus</em>, <em>Inquilinus</em>, <em>Gracilibacillus</em>, <em>Metabacillus</em>, <em>Neobacillus</em>, <em>Paenibacillus</em>, <em>Priestia</em>, <em>Staphylococcus</em>, <em>Streptomyces</em> and <em>Virgibacillus</em> were among the identified genera. The antifungal potential of the culture supernatant (CS) of 39 halophilic bacteria was investigated against <em>C</em>. <em>gloeosporioides</em> and <em>A</em>. <em>alternata</em>. The bacteria showing the greatest inhibition of mycelial growth corresponded to <em>Bacillus subtilis</em> CPO 4292, <em>Metabacillus</em> sp. CPO 4266, <em>Bacillus</em> sp. CPO 4295 and <em>Bacillus</em> sp. CPO 4279. The CS of <em>Bacillus</em> sp. CPO 4279 exhibited the highest activity and its ethyl acetate extract (AcOEt) inhibited the germination of <em>C</em>. <em>gloeosporioides</em>, with IC<sub>50</sub> values of 8,630<!--> <!-->μg/ml and IC<sub>90</sub> of 10,720<!--> <!-->μg/ml. The organic partition of the AcOEt extract led to three fractions, with acetonitrile (FAcB9) showing the highest antifungal activity, with values exceeding 66%. Halophilic bacteria from ‘blanquizales’ soils of the genus <em>Bacillus</em> sp. produce metabolites with antifungal properties that inhibit the phytopathogenic fungus <em>C</em>. <em>gloeosporioides</em>.</p></div>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754124000233/pdfft?md5=9262279ae0a4f9cff2fa928452c96f9c&pid=1-s2.0-S0325754124000233-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0325754124000233","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Phytopathogenic fungi Alternaria alternata and Colletotrichum gloeosporioides cause diseases in plant tissues as well as significant postharvest losses. The use of chemical fungicides for their control has negative effects on health and the environment. Secondary metabolites from halophilic bacteria are a promising alternative for new antifungal compounds. In the present study, halophilic bacteria were isolated and characterized from two sites with saline soils called branquizales in Campeche, Mexico. A total of 64 bacteria were isolated. Agrobacterium, Bacillus, Inquilinus, Gracilibacillus, Metabacillus, Neobacillus, Paenibacillus, Priestia, Staphylococcus, Streptomyces and Virgibacillus were among the identified genera. The antifungal potential of the culture supernatant (CS) of 39 halophilic bacteria was investigated against C. gloeosporioides and A. alternata. The bacteria showing the greatest inhibition of mycelial growth corresponded to Bacillus subtilis CPO 4292, Metabacillus sp. CPO 4266, Bacillus sp. CPO 4295 and Bacillus sp. CPO 4279. The CS of Bacillus sp. CPO 4279 exhibited the highest activity and its ethyl acetate extract (AcOEt) inhibited the germination of C. gloeosporioides, with IC50 values of 8,630 μg/ml and IC90 of 10,720 μg/ml. The organic partition of the AcOEt extract led to three fractions, with acetonitrile (FAcB9) showing the highest antifungal activity, with values exceeding 66%. Halophilic bacteria from ‘blanquizales’ soils of the genus Bacillus sp. produce metabolites with antifungal properties that inhibit the phytopathogenic fungus C. gloeosporioides.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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