{"title":"Disruption of RGD1 Gene Improves Acetic Acid Tolerance in Saccharomyces cerevisiae","authors":"H. S. Kim","doi":"10.1134/S000368382402008X","DOIUrl":null,"url":null,"abstract":"<p>This study aimed to identify genes related to acetic acid tolerance and elucidate the tolerance mechanism in yeast <i>Saccharomyces cerevisiae</i> as an appropriate eukaryote model. In this study, one tolerant strain exposed to acetic acid stress was isolated by screening a transposon-mediated mutant library and the disrupted gene was identified to be <i>RGD1</i>. In addition, this phenotype related to acetic acid tolerance was confirmed by deletion and overexpressing of this corresponding gene. This mutant strain showed activation of Hog1p and increased expression of the <i>MSN2/4</i> and STRE-mediated genes such as <i>CTT1</i> and <i>HSP12</i> in response to acetic acid stress.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 2","pages":"294 - 300"},"PeriodicalIF":1.0000,"publicationDate":"2024-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S000368382402008X","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
This study aimed to identify genes related to acetic acid tolerance and elucidate the tolerance mechanism in yeast Saccharomyces cerevisiae as an appropriate eukaryote model. In this study, one tolerant strain exposed to acetic acid stress was isolated by screening a transposon-mediated mutant library and the disrupted gene was identified to be RGD1. In addition, this phenotype related to acetic acid tolerance was confirmed by deletion and overexpressing of this corresponding gene. This mutant strain showed activation of Hog1p and increased expression of the MSN2/4 and STRE-mediated genes such as CTT1 and HSP12 in response to acetic acid stress.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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