Basal differentiation and expression status of SOX2 and KLF4 in basal layers are prognostic factors for disease-specific survival in oral squamous cell carcinoma

IF 2.7 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of Oral Pathology & Medicine Pub Date : 2024-04-24 DOI:10.1111/jop.13535

Kazuhiro Murakami, Tomoko Kamiakito, Hirotoshi Kawata, Koki Kosami, Takeo Nakaya, Tadahide Noguchi, Yoshiyuki Mori, Akira Tanaka

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Abstract

Background

Basal differentiation in oral squamous cell carcinoma is usually detected at invasive sites. However, its significance as a prognostic value has been poorly investigated.

Methods

COL17 was selected as a basal differentiation marker because of its stable expression in the basal-like cells of oral squamous cell carcinoma. Sixty-five cases of oral squamous cell carcinoma were subclassified into COL17-high (30 cases) and -low (35 cases) types, and the prognostic value was analyzed by Cox regression analysis. In addition, the stem cell markers such as SOX2, KLF4, MYC as well as the stem cell-related markers BMI1, EZH2, and YAP and its paralog TAZ, were immunohistochemically analyzed. Their prognostic values were investigated along with their COL17 status by Cox regression analysis.

Results

No significant difference was observed between the COL17-high and -low groups in the disease-specific survival and recurrence-free survival in oral squamous cell carcinoma. When the COL17-high and -low categories were combined with the SOX2, KLF4, EZH2, or YAP/TAZ status in the basal layers, together with gender and age as covariates, the hazard ratios reached 3.3, 3.7, 2.8, and 3.1, respectively. In addition, multivariate analysis, including COL17, SOX2, and KLF4, with gender and age as covariates, showed a significantly poor prognosis for disease-specific survival.

Conclusion

Based on the relatively high hazard ratios, it is indicated that basal differentiation and the expression status of SOX2 and KLF4 in the basal layers are prognostic factors for oral squamous cell carcinoma.

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基底分化以及基底层 SOX2 和 KLF4 的表达状态是口腔鳞状细胞癌疾病特异性生存率的预后因素

背景口腔鳞状细胞癌的基底分化通常在浸润部位检测到。由于 COL17 在口腔鳞状细胞癌的基底样细胞中稳定表达，因此被选为基底分化标志物。将 65 例口腔鳞状细胞癌细分为 COL17 高（30 例）和低（35 例）两型，并通过 Cox 回归分析分析其预后价值。此外，还对干细胞标记物，如 SOX2、KLF4、MYC 以及干细胞相关标记物 BMI1、EZH2 和 YAP 及其旁系亲属 TAZ 进行了免疫组化分析。结果在口腔鳞状细胞癌的疾病特异性生存率和无复发生存率方面，COL17 高组和低组间未观察到显著差异。将 COL17 高、低两组与基底层的 SOX2、KLF4、EZH2 或 YAP/TAZ 状态相结合，再加上性别和年龄作为协变量，危险比分别达到 3.3、3.7、2.8 和 3.1。此外，包括 COL17、SOX2 和 KLF4 在内的多变量分析以及性别和年龄作为协变量，均显示疾病特异性生存的预后明显较差。

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来源期刊

Journal of Oral Pathology & Medicine 医学-病理学

CiteScore

5.90

自引率

6.10%

发文量

121

审稿时长

4-8 weeks

期刊介绍： The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.