In-vitro Dilution of Human Plasma to Reduce ABO Antibody Titer Levels While Preserving Coagulation Factors

IF 0.9 4区医学 Indian Journal of Hematology and Blood Transfusion Pub Date : 2024-04-27 DOI:10.1007/s12288-024-01784-0

Seema Kumari Meena, Gopal Kumar Patidar, R. Lakshmy, Rahul Chaurasia, Anjali Hazarika

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Abstract

The objective of this study was to prepare universal plasma by mixing Group A and Group B plasma with normal saline in varying proportions. This prospective in vitro study involved mixing blood group A and B plasma in ratios of 1:1, 3:1, 1:3, and with normal saline in ratios of 1:1:1, 2:2:1, and 1:1:2. The titration of IgG and IgM levels of anti-A and anti-B antibodies, as well as fibrinogen and factor VIII levels, were performed. The titers of anti-A and anti-B antibodies of both IgG and IgM types were found to have significantly decreased in all ratios compared to the control sample. Coagulation factors were found to be higher than the allowable limit when simple plasma mixing ratios (1:1, 3:1, and 1:3) were used, while they significantly decreased upon dilution with normal saline in 1:1:1 and 1:1:2 ratios. In conclusion, the mixing of A and B blood group plasma at a fixed ratio may be a promising strategy for the preparation of universal plasma with a low titre of haemolytic antibodies, while maintaining an adequate amount of coagulation factors.

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体外稀释人血浆以降低 ABO 抗体滴度水平，同时保留凝血因子

摘要本研究的目的是通过将 A 组血浆和 B 组血浆与生理盐水按不同比例混合来制备通用血浆。这项前瞻性体外研究将 A 组和 B 组血浆按 1:1、3:1、1:3 的比例混合，并与正常生理盐水按 1:1:1、2:2:1 和 1:1:2 的比例混合。对抗 A 和抗 B 抗体的 IgG 和 IgM 水平以及纤维蛋白原和因子 VIII 水平进行滴定。结果发现，与对照样本相比，IgG 和 IgM 型抗 A 和抗 B 抗体的滴度在所有比例下都明显下降。在使用简单的血浆混合比例（1:1、3:1 和 1:3）时，凝血因子高于允许限度，而在使用正常生理盐水稀释 1:1:1 和 1:1:2 比例时，凝血因子则明显降低。总之，以固定比例混合 A 和 B 血型血浆可能是制备溶血抗体滴度较低的通用血浆的一种有前途的策略，同时还能保持足够数量的凝血因子。

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Indian Journal of Hematology and Blood Transfusion Medicine-Hematology

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期刊介绍： Indian Journal of Hematology and Blood Transfusion is a medium for propagating and exchanging ideas within the medical community. It publishes peer-reviewed articles on a variety of aspects of clinical hematology, laboratory hematology and hemato-oncology. The journal exists to encourage scientific investigation in the study of blood in health and in disease; to promote and foster the exchange and diffusion of knowledge relating to blood and blood-forming tissues; and to provide a forum for discussion of hematological subjects on a national scale. The Journal is the official publication of The Indian Society of Hematology & Blood Transfusion.