Genome assembly and annotation of Spilosoma obliqua multicapsid nucleopolyhedrovirus from Bihar hairy caterpillar, an agriculturally important insect pest

IF 1.1 4区农林科学 Q3 PLANT SCIENCES Journal of Phytopathology Pub Date : 2024-05-03 DOI:10.1111/jph.13308

Mohammad Akram, Mir Asif Iquebal, Kiran Gandhi Baptala, Sarika Jaiswal, Sujayanand Gopalakrishnan Kesharivarmen, Naimuddin Kamaal, Jaisri J, Abhishek Bohra, Khelaram Soren, Deepak Kumar Sachan, Raja Husain, Deepender Kumar, Anil Rai, Dinesh Kumar

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Abstract

Spilosoma obliqua nucleopolyhedrovirus (SpobNPV) is known as a biocontrol agent against S. obliqua a polyphagous insect. Genome of an isolate designated as SpobMNPV was sequenced and found to have 136,141 bp, 139 putative open reading frames (ORFs) on both sense (48%) and anti-sense (52%) strands and 97.91% nucleotide similarity with Hyphantria cunea nucleopolyhedrovirus (HycuNPV). All the 38 core genes of baculoviruses were identified and validated in the SpobMNPV genome, which differed from SpobNPV–Manipur isolate in several aspects. In SpobMNPV genome, 7 h were found comprised of 2 to 16 repeated units of 67-bp at each site with an imperfect 30-bp palindrome near the centre in both orientations. Comparison of consensus palindrome sequences (hrcons) present in hrs with that of selected alphabaculovirus group I NPVs revealed them to be completely conserved at each side of the hrcons, that is 1-GxTTTxC-7 and 22-TxGxAAAxC-30. Based on phylogenetic analysis of 38 core genes, SpobMNPV was found closest to the HycuNPV in the group I alphabaculovirus. The complete genome of this isolate is being reported for the first time from North India. The information on genome analysis of SpobMNPV will be an addition to the available database on alphabaculoviruses and also accelerate research on SpobNPV as a component of integrated management of S. obliqua in many economically important crops.

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比哈尔毛毛虫--一种重要的农业害虫--的 Spilosoma obliqua 多囊核多面体病毒的基因组组装和注释

据了解，Spilosoma obliqua nucleopolyhedrovirus（SpobNPV）是一种针对多食性昆虫 S. obliqua 的生物控制剂。对一个被命名为 SpobMNPV 的分离株的基因组进行了测序，发现该分离株的基因组有 136 141 bp，139 个推测开放阅读框（ORF）分别位于有义链（48%）和无义链（52%）上，与 Hyphantria cunea nucleopolyhedrovirus（HycuNPV）的核苷酸相似度为 97.91%。在 SpobMNPV 基因组中鉴定并验证了所有 38 个杆状病毒核心基因，它们与 SpobNPV-Manipur 分离物在多个方面存在差异。在 SpobMNPV 基因组中，发现有 7 个 h 由 2 至 16 个重复单位组成，每个位点有 67 个 bp，在两个方向的中心附近都有一个不完全的 30 个 bp 的回文键。将 hrs 中存在的共识回文序列（hrcons）与所选的字母病毒 I 组 NPV 的序列进行比较，发现它们在 hrcons 的两侧完全一致，即 1-GxTTTxC-7 和 22-TxGxAAAxC-30。根据对 38 个核心基因的系统进化分析，发现 SpobMNPV 与 I 组字母杆状病毒中的 HycuNPV 最为接近。该分离株的完整基因组是首次从北印度报告。有关 SpobMNPV 基因组分析的信息将为现有的 alphabaculovirus 数据库增添新的内容，同时也将加速 SpobNPV 的研究，因为 SpobNPV 是许多重要经济作物上 S. obliqua 综合治理的一个组成部分。

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来源期刊

Journal of Phytopathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.