Comparison of skeletal muscle decellularization protocols and recellularization with adipose-derived stem cells for tissue engineering

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-05-01 DOI:10.1016/j.biologicals.2024.101767

Joyce Esposito , Pricila da Silva Cunha , Thaís Maria da Mata Martins , Mariane Izabella Abreu de Melo , Marcos Augusto de Sá , Dawidson Assis Gomes , Alfredo Miranda de Góes

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Abstract

Decellularization is a novel technique employed for scaffold manufacturing, as a strategy for skeletal muscle (SM) tissue engineering applications. However, poor decellularization efficacy is still a problem for the use of decellularized scaffolds as truly biocompatible biomaterials. For recellularization, adipose-derived stem cells (ASCs) are a good option, due to their immunomodulatory and pro-regenerative capacity, but few studies have described their combination with muscle-decellularized matrices (mDMs). This work aimed to evaluate the efficiency of four multi-step decellularization protocols to produce mDMs and to investigate in vitro biocompatibility with ASCs. Here, we described the different efficacies of muscle decellularization methods, suggesting the need for stricter standardization of the method, considering the large range of applications in SM tissue engineering, which is also a promising platform for preclinical studies with rat disease models using autologous cells.

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比较用于组织工程的骨骼肌脱细胞方案和脂肪干细胞再细胞化方案

脱细胞是一种新型的支架制造技术，是骨骼肌（SM）组织工程应用的一种策略。然而，脱细胞效果不佳仍然是将脱细胞支架用作真正生物相容性生物材料的一个问题。对于再细胞化，脂肪衍生干细胞（ASCs）因其免疫调节和促进再生的能力而成为一个不错的选择，但很少有研究描述其与肌肉脱细胞基质（mDMs）的结合。这项工作旨在评估四种多步骤脱细胞方案生产 mDMs 的效率，并研究 ASCs 的体外生物相容性。在这里，我们描述了肌肉脱细胞方法的不同功效，考虑到SM组织工程的广泛应用，我们认为有必要对该方法进行更严格的标准化，这也是使用自体细胞对大鼠疾病模型进行临床前研究的一个很有前景的平台。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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