Influences of Ipomoea batatas Anti-Cancer Peptide on Tomato Defense Genes

IF 1.9 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Current protein & peptide science Pub Date : 2024-05-03 DOI:10.2174/0113892037299818240408053000

Hsin-Hung Lin, Kuan-Hung Lin, Yung-Lin Tsai, Rong-Jane Chen, Yen-Chang Lin, Yu-Chi Chen

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Abstract

Aims: This study investigates the impact of IbACP (Ipomoea batatas anti-cancer peptide) on defense-related gene expression in tomato leaves, focusing on its role in plant defense mechanisms. Background: IbACP was isolated from sweet potato leaves, and it was identified as a peptide capable of inducing an alkalinization response in tomato suspension culture media. Additionally, IbACP was found to regulate the proliferation of human pancreatic adenocarcinoma cells. Objective: Elucidate IbACP's molecular influence on defense-related gene expression in tomato leaves using next-generation sequencing analysis. method: To assess the impact of IbACP on defense-related gene expression, transcriptome data were analyzed, encompassing various functional categories such as photosynthesis, metabolic processes, and plant defense. Semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) analysis was employed to verify transcription levels of defense-related genes in tomato leaves treated with IbACP. Method: To assess the impact of IbACP on defense-related gene expression, transcriptome data were analyzed, encompassing various functional categories such as photosynthesis, metabolic processes, and plant defense. Semi-quantitative reverse-transcription polymerase chain reaction analysis was employed to verify transcription levels of defense-related genes in tomato leaves treated with IbACP for durations ranging from 0 h (control) to 24 h. Results: IbACP induced jasmonic acid-related genes (LoxD and AOS) at 2 h, with a significant up-regulation of salicylic acid-dependent gene NPR1 at 24 h. This suggested a temporal antagonistic effect between jasmonic acid and salicylic acid during the early hours of IbACP treatment. Downstream ethylene-responsive regulator genes (ACO1, ETR4, and ERF1) were consistently down-regulated by IbACP at all times. Additionally, IbACP significantly up-regulated the gene expressions of suberization-associated anionic peroxidases (TMP1 and TAP2) at all time points, indicating enhanced suberization of the plant cell wall to prevent pathogen invasion. Conclusion: IbACP enhances the synthesis of defense hormones and up-regulates downstream defense genes, improving the plant's resistance to biotic stresses.

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Ipomoea batatas 抗癌肽对番茄防御基因的影响

目的：本研究调查了 IbACP（Ipomoea batatas 抗癌肽）对番茄叶片防御相关基因表达的影响，重点研究其在植物防御机制中的作用。背景：IbACP 从甘薯叶片中分离出来，经鉴定，它是一种能诱导番茄悬浮培养基碱化反应的多肽。此外，研究还发现 IbACP 能调节人类胰腺癌细胞的增殖。研究目的利用新一代测序分析，阐明 IbACP 对番茄叶片中防御相关基因表达的分子影响：为了评估 IbACP 对防御相关基因表达的影响，分析了包括光合作用、代谢过程和植物防御等各种功能类别的转录组数据。采用半定量反转录聚合酶链反应（RT-PCR）分析来验证 IbACP 处理的番茄叶片中防御相关基因的转录水平。方法：为了评估 IbACP 对防御相关基因表达的影响，分析了包括光合作用、代谢过程和植物防御等各种功能类别的转录组数据。采用半定量反转录聚合酶链反应分析法验证了番茄叶片在 IbACP 处理 0 小时（对照）至 24 小时期间防御相关基因的转录水平：这表明在 IbACP 处理的早期，茉莉酸和水杨酸之间存在时间上的拮抗作用。乙烯反应调控因子的下游基因（ACO1、ETR4 和 ERF1）在所有时间段都被 IbACP 一致下调。此外，IbACP 在所有时间点都显著上调了与琥珀化相关的阴离子过氧化物酶（TMP1 和 TAP2）的基因表达，这表明植物细胞壁的琥珀化作用增强，可防止病原体入侵。结论IbACP 可增强防御激素的合成并上调下游防御基因，从而提高植物对生物胁迫的抵抗力。

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来源期刊

Current protein & peptide science 生物-生化与分子生物学

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Current Protein & Peptide Science publishes full-length/mini review articles on specific aspects involving proteins, peptides, and interactions between the enzymes, the binding interactions of hormones and their receptors; the properties of transcription factors and other molecules that regulate gene expression; the reactions leading to the immune response; the process of signal transduction; the structure and function of proteins involved in the cytoskeleton and molecular motors; the properties of membrane channels and transporters; and the generation and storage of metabolic energy. In addition, reviews of experimental studies of protein folding and design are given special emphasis. Manuscripts submitted to Current Protein and Peptide Science should cover a field by discussing research from the leading laboratories in a field and should pose questions for future studies. Original papers, research articles and letter articles/short communications are not considered for publication in Current Protein & Peptide Science.