Assessment of the association of myofibroblasts and structural components of the extracellular matrix with histopathological parameters of actinic cheilitis and lower lip squamous cell carcinoma

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-05-01 DOI:10.1111/jop.13541

Farah Essgui Orellana Martinez, Thâmara Manoela Marinho Bezerra, Ana Paula Negreiros Nunes Alves, Isabelle Joyce Lima Silva Fernandes, Fabricio Bitu Sousa, Paulo Goberlânio de Barros Silva, Mário Rogério Lima Mota

{"title":"Assessment of the association of myofibroblasts and structural components of the extracellular matrix with histopathological parameters of actinic cheilitis and lower lip squamous cell carcinoma","authors":"Farah Essgui Orellana Martinez, Thâmara Manoela Marinho Bezerra, Ana Paula Negreiros Nunes Alves, Isabelle Joyce Lima Silva Fernandes, Fabricio Bitu Sousa, Paulo Goberlânio de Barros Silva, Mário Rogério Lima Mota","doi":"10.1111/jop.13541","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>To evaluate the presence of myofibroblasts (MFs) in the development of lip carcinogenesis, through the correlation of clinical, histomorphometric and immunohistochemical parameters, in actinic cheilitis (ACs) and lower lip squamous cell carcinomas (LLSCCs).</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Samples of ACs, LLSCCs, and control group (CG) were prepared by tissue microarray (TMA) for immunohistochemical TGF-β, α-SMA, and Ki-67 and histochemical hematoxylin and eosin, picrosirius red, and verhoeff van gieson reactions. Clinical and microscopic data were associated using the Mann–Whitney, Kruskal-Wallis/Dunn, and Spearman correlation tests (SPSS, <i>p</i> < 0.05).</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>ACs showed higher number of α-SMA<sup>+</sup> MFs when compared to CG (<i>p</i> = 0.034), and these cells were associated with the vertical expansion of solar elastosis (SE) itself (<i>p</i> = 0.027). Areas of SE had lower deposits of collagen (<i>p</i> < 0.001), immunostaining for TGF-β (<i>p</i> < 0.001), and higher density of elastic fibers (<i>p</i> < 0.05) when compared to areas without SE. A positive correlation was observed between high-risk epithelial dysplasia (ED) and the proximity of SE to the dysplastic epithelium (<i>p</i> = 0.027). LLSCCs showed a higher number of α-SMA<sup>+</sup> MFs about CG (<i>p</i> = 0.034), as well as a reduction in the deposition of total collagen (<i>p</i> = 0.009) in relation to ACs and CG. There was also a negative correlation between the amount of α-SMA<sup>+</sup> cells and the accumulation of total collagen (<i>p</i> = 0.041). Collagen and elastic density loss was higher in larger tumors (<i>p</i> = 0.045) with nodal invasion (<i>p</i> = 0.047).</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Our findings show the possible role of MFs, collagen fibers, and elastosis areas in the lip carcinogenesis process.</p>\n </section>\n </div>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jop.13541","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}

引用次数: 0

Abstract

Background

To evaluate the presence of myofibroblasts (MFs) in the development of lip carcinogenesis, through the correlation of clinical, histomorphometric and immunohistochemical parameters, in actinic cheilitis (ACs) and lower lip squamous cell carcinomas (LLSCCs).

Methods

Samples of ACs, LLSCCs, and control group (CG) were prepared by tissue microarray (TMA) for immunohistochemical TGF-β, α-SMA, and Ki-67 and histochemical hematoxylin and eosin, picrosirius red, and verhoeff van gieson reactions. Clinical and microscopic data were associated using the Mann–Whitney, Kruskal-Wallis/Dunn, and Spearman correlation tests (SPSS, p < 0.05).

Results

ACs showed higher number of α-SMA⁺ MFs when compared to CG (p = 0.034), and these cells were associated with the vertical expansion of solar elastosis (SE) itself (p = 0.027). Areas of SE had lower deposits of collagen (p < 0.001), immunostaining for TGF-β (p < 0.001), and higher density of elastic fibers (p < 0.05) when compared to areas without SE. A positive correlation was observed between high-risk epithelial dysplasia (ED) and the proximity of SE to the dysplastic epithelium (p = 0.027). LLSCCs showed a higher number of α-SMA⁺ MFs about CG (p = 0.034), as well as a reduction in the deposition of total collagen (p = 0.009) in relation to ACs and CG. There was also a negative correlation between the amount of α-SMA⁺ cells and the accumulation of total collagen (p = 0.041). Collagen and elastic density loss was higher in larger tumors (p = 0.045) with nodal invasion (p = 0.047).

Conclusions

Our findings show the possible role of MFs, collagen fibers, and elastosis areas in the lip carcinogenesis process.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

评估肌成纤维细胞和细胞外基质结构成分与口角炎和下唇鳞状细胞癌组织病理学参数的关系

背景通过临床、组织形态学和免疫组化参数的相关性，评估肌成纤维细胞（MFs）在动胬肉（ACs）和下唇鳞状细胞癌（LLSCCs）唇癌发生过程中的存在情况。方法用组织芯片（TMA）制备下唇鳞状细胞癌（ACs）、下唇鳞状细胞癌（LLSCCs）和对照组（CG）的样本，进行免疫组化 TGF-β、α-SMA 和 Ki-67，以及组织化学苏木精和伊红、苦橙红和verhoeff van gieson 反应。采用 Mann-Whitney、Kruskal-Wallis/Dunn 和 Spearman 相关性检验（SPSS，p <0.05）对临床和显微镜数据进行关联检验。与没有出现日光性弹力纤维症的区域相比，出现日光性弹力纤维症的区域胶原蛋白沉积较低（p = 0.001），TGF-β免疫染色较低（p = 0.001），弹力纤维密度较高（p = 0.05）。在高风险上皮发育不良（ED）与SE与发育不良上皮的邻近程度之间观察到正相关（p = 0.027）。与 AC 和 CG 相比，LLSCC 显示出更多的α-SMA+ MFs（p = 0.034），以及总胶原沉积的减少（p = 0.009）。α-SMA+细胞的数量与总胶原蛋白的积累之间也呈负相关（p = 0.041）。结论我们的研究结果表明，中频、胶原纤维和弹性区域在唇癌发生过程中可能起着重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊