Magnolia biondii flower extract attenuates UVB-induced skin damage through high-mobility group box protein B1

IF 2.7 4区医学 Q2 DERMATOLOGY International Journal of Cosmetic Science Pub Date : 2024-04-29 DOI:10.1111/ics.12959

Fang Huang, Qing Liu, Yina Lu

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Abstract

Objective

Magnolia biondii, a plant containing many magnolian-like compounds in its flowers or buds, exhibits anti-inflammatory and antiallergic effects; however, no study has addressed its effect on alleviating ultraviolet light (UV)-induced skin damage. We thus aimed at studying the effects of M. biondii flower extract (MB) on UVB-induced skin damage and determine the relationship between cell damage and damage-associated molecular patterns (DAMPs).

Methods

Reconstructed epidermal models and foreskin samples were selected to detect cellular reactions after UVB irradiation and MB treatment. MTT, haematoxylin–eosin and immunofluorescence staining were used to examine total viability, sunburned cells and expression and migration of DAMPs at 16 or 48 h. Prostaglandin E2 (PGE-2) and interleukin 8 (IL-8) levels were measured using enzyme-linked immunosorbent assays. A clinical UVB-damaged test was carried out on human arms subjected to MB pre- or post-treatment. Human skin probes were used to measure erythema, melanin, ITA° and transepidermal water loss (TEWL), while skin photos were captured using the VISIA system.

Results

MB is rich in lignans such as magnolin, pinoresinol dimethyl ether and fargesin, and shows weak UV absorption at 280–320 nm. Coculturing with MB for 16 or 48 h after UVB irradiation improved the tissue viability and structure of Skinovo-Epi, and reduced the expression and migration of high mobility group box protein B1 (HMGB1) as well as the expression of IL-8 and PGE-2. In the excised foreskin treated with MB after UVB irradiation, the generation of 8-hidroxy-2-deoxyguanosine and nuclear transfer of HMGB1 were reduced. When pre-treated with MB for 3 days, UVB-induced skin erythema and ITA° were significantly decreased. When post-treated with MB for 5 days, a decrease in skin erythema, melanin and TEWL values and an increase in skin ITA° were observed.

Conclusions

Treatment with MB attenuated UVB-induced skin damage, such as erythema, pigmentation and skin barrier function, by improving the tissue viability and structure and reducing sunburned cells and skin inflammation. This effect may be related to DNA damage, which causes the migration of HMGB1 from the nucleus to the outside of the cell to induce skin inflammation.

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木兰花萃取物通过高迁移率基团盒蛋白 B1 减轻紫外线诱导的皮肤损伤

目的厚朴（Magnolia biondii）是一种在花朵或花蕾中含有多种厚朴类化合物的植物，具有抗炎和抗过敏的作用；但是，还没有研究探讨过厚朴对减轻紫外线（UV）诱导的皮肤损伤的作用。因此，我们旨在研究 M. biondii 花提取物（MB）对紫外线诱导的皮肤损伤的影响，并确定细胞损伤与损伤相关分子模式（DAMPs）之间的关系。用 MTT、血色素-伊红和免疫荧光染色法检测 16 或 48 小时后的总存活率、晒伤细胞以及 DAMPs 的表达和迁移情况；用酶联免疫吸附测定法测量前列腺素 E2（PGE-2）和白细胞介素 8（IL-8）的水平。对接受甲基溴治疗前或治疗后的人体手臂进行了临床紫外线损伤测试。使用人体皮肤探针测量红斑、黑色素、ITA°和经表皮失水（TEWL），同时使用 VISIA 系统拍摄皮肤照片。ResultsMB 富含木脂素，如 magnolin、pinoresinol dimethyl ether 和 fargesin，在 280-320 纳米波长处对紫外线有弱吸收。在 UVB 照射后与 MB 共同培养 16 或 48 小时，可提高 Skinovo-Epi 的组织活力和结构，减少高迁移率基团盒蛋白 B1（HMGB1）的表达和迁移，以及 IL-8 和 PGE-2 的表达。在 UVB 照射后用甲基溴处理的切除包皮中，8-羟基-2-脱氧鸟苷的生成和 HMGB1 的核转移均有所减少。用甲基溴预处理 3 天后，紫外线诱发的皮肤红斑和 ITA°明显减少。结论用甲基溴治疗可减轻 UVB 引起的皮肤损伤，如红斑、色素沉着和皮肤屏障功能，改善组织活力和结构，减少晒伤细胞和皮肤炎症。这种效应可能与 DNA 损伤有关，DNA 损伤会导致 HMGB1 从细胞核向细胞外迁移，从而诱发皮肤炎症。

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来源期刊

International Journal of Cosmetic Science DERMATOLOGY-

CiteScore

4.60

自引率

4.30%

发文量

期刊介绍： The Journal publishes original refereed papers, review papers and correspondence in the fields of cosmetic research. It is read by practising cosmetic scientists and dermatologists, as well as specialists in more diverse disciplines that are developing new products which contact the skin, hair, nails or mucous membranes. The aim of the Journal is to present current scientific research, both pure and applied, in: cosmetics, toiletries, perfumery and allied fields. Areas that are of particular interest include: studies in skin physiology and interactions with cosmetic ingredients, innovation in claim substantiation methods (in silico, in vitro, ex vivo, in vivo), human and in vitro safety testing of cosmetic ingredients and products, physical chemistry and technology of emulsion and dispersed systems, theory and application of surfactants, new developments in olfactive research, aerosol technology and selected aspects of analytical chemistry.