Comparison of peripheral blood automated hematopoietic progenitor cell count and flow cytometric CD34+ cell count

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-05-06 DOI:10.1002/jca.22114

Mischa Hagenkötter, Thomas Mika, Swetlana Ladigan-Badura, Karin Schork, Martin Eisenacher, Roland Schroers, Alexander Baraniskin

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Abstract

Background

Stem cell apheresis in the context of autologous stem cell transplantation requires an accurate cluster of differentiantion 34 (CD34⁺) count determined by flow cytometry as the current gold standard. Since flow cytometry is a personnel and time-intensive diagnostic tool, automated stem cell enumeration may provide a promising alternative. Hence, this study aimed to compare automated hematopoietic progenitor enumeration carried out on a Sysmex XN-20 module compared with conventional flow cytometric measurements.

Methods

One hundred forty-three blood samples from 41 patients were included in this study. Correlation between the two methods was calculated over all samples, depending on leukocyte count and diagnosis.

Results

Overall, we found a high degree of correlation (r = 0.884). Furthermore, correlation was not impaired by elevated leukocyte counts (>10 000/μL, r = 0.860 vs <10 000/μL, r = 0.849; >20 000/μL, r = 0.843 vs <20 000/μL, r = 0.875). However, correlation was significantly impaired in patients with multiple myeloma (multiple myeloma r = 0.840 vs nonmyeloma r = 0.934).

Summary

Stem cell measurement carried out on the Sysmex XN-20 module provides a significant correlation with flow cytometry and might be implemented in clinical practice. In clinical decision-making, there was discrepancy of under 15% of cases. In multiple myeloma patients, XN-20 should be used with caution.

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外周血自动造血祖细胞计数与流式细胞仪 CD34+ 细胞计数的比较

背景在自体干细胞移植的背景下，干细胞分离需要以流式细胞术作为目前的黄金标准，确定精确的分化簇34（CD34+）计数。由于流式细胞术是一种人员和时间密集型诊断工具，自动干细胞计数可能提供一种有前途的替代方法。因此，本研究旨在比较使用Sysmex XN-20模块进行的自动造血祖细胞计数与传统流式细胞术测量。方法本研究纳入了 41 名患者的 143 份血液样本。根据白细胞计数和诊断结果，计算了所有样本中两种方法的相关性。结果总体而言，我们发现了高度的相关性（r = 0.884）。此外，白细胞计数升高不会影响相关性（>10 000/μL, r = 0.860 vs <10 000/μL, r = 0.849; >20 000/μL, r = 0.843 vs <20 000/μL, r = 0.875）。然而，多发性骨髓瘤患者的相关性明显降低（多发性骨髓瘤 r = 0.840 vs 非骨髓瘤 r = 0.934）。小结在 Sysmex XN-20 模块上进行的干细胞测量与流式细胞术具有明显的相关性，可用于临床实践。在临床决策中，15%以下的病例存在差异。多发性骨髓瘤患者应慎用 XN-20。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.