Turbidimetric bioassays: A solution to antimicrobial activity detection in asymptomatic bacteriuria isolates against uropathogenic Escherichia coli

IF 3.9 3区 生物学 Q2 MICROBIOLOGY MicrobiologyOpen Pub Date : 2024-05-06 DOI:10.1002/mbo3.1411
Ciara Kenneally, Craig P. Murphy, Roy D. Sleator, Eamonn P. Culligan
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Abstract

Traditional bacteriocin screening methods often face limitations due to diffusion-related challenges in agar matrices, which can prevent the peptides from reaching their target organism. Turbidimetric techniques offer a solution to these issues, eliminating diffusion-related problems and providing an initial quantification of bacteriocin efficacy in producer organisms. This study involved screening the cell-free supernatant (CFS) from eight uncharacterized asymptomatic bacteriuria (ABU) isolates and Escherichia coli 83972 for antimicrobial activity against clinical uropathogenic E. coli (UPEC) strains using turbidimetric growth methods. ABU isolates exhibiting activity against five or more UPEC strains were further characterized (PUTS 37, PUTS 58, PUTS 59, S-07-4, and SK-106-1). The inhibition of the CFS by proteinase K suggested that the antimicrobial activity was proteinaceous in nature, potentially bacteriocins. The activity of E. coli PUTS 58 and SK-106-1 was enhanced in an artificial urine medium, with both inhibiting all eight UPECs. A putative microcin H47 operon was identified in E. coli SK-106-1, along with a previously identified microcin V and colicin E7 in E. coli PUTS 37 and PUTS 58, respectively. These findings indicate that ABU bacteriocin-producers could serve as viable prophylactics and therapeutics in the face of increasing antibiotic resistance among uropathogens.

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比浊生物测定法:无症状菌尿分离物对尿路致病性大肠杆菌抗菌活性检测的解决方案
传统的细菌素筛选方法往往受到琼脂基质中与扩散有关的难题的限制,这些难题会阻碍肽到达目标生物体。比浊技术为这些问题提供了一种解决方案,它消除了与扩散相关的问题,并能初步量化细菌素在生产者生物体中的效力。本研究采用浊度生长法对来自 8 个未定性无症状菌尿症(ABU)分离物和大肠杆菌 83972 的无细胞上清液(CFS)进行筛选,以确定其对临床尿路致病性大肠杆菌(UPEC)菌株的抗菌活性。对五株或五株以上 UPEC 菌株具有活性的 ABU 分离物(PUTS 37、PUTS 58、PUTS 59、S-07-4 和 SK-106-1)被进一步鉴定。蛋白酶 K 对 CFS 的抑制作用表明,其抗菌活性本质上是蛋白性的,可能是细菌素。在人工尿培养基中,大肠杆菌 PUTS 58 和 SK-106-1 的活性增强,两者都能抑制所有八种 UPECs。在大肠杆菌 SK-106-1 中发现了一个推定的微素 H47 操作子,以及之前在大肠杆菌 PUTS 37 和 PUTS 58 中分别发现的微素 V 和大肠杆菌素 E7。这些研究结果表明,面对泌尿道病原体对抗生素的耐药性不断增加,ABU 细菌素生产者可作为可行的预防和治疗药物。
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来源期刊
MicrobiologyOpen
MicrobiologyOpen MICROBIOLOGY-
CiteScore
8.00
自引率
0.00%
发文量
78
审稿时长
20 weeks
期刊介绍: MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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