Dexmedetomidine improves lung injury after one-lung ventilation in esophageal cancer patients by inhibiting inflammatory response and oxidative stress.

IF 2.2 4区 医学 Q3 TOXICOLOGY Toxicology Research Pub Date : 2024-04-10 eCollection Date: 2024-04-01 DOI:10.1093/toxres/tfae041
Zhen Liu, Yingfen Xiong, Jia Min, Yunsheng Zhu
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Abstract

Aim: To explore the effect of Dexmedetomidine (DEX) on lung injury in patients undergoing One-lung ventilation (OLV).

Methods: Esophageal cancer patients undergoing general anesthesia with OLV were randomly divided into the DEX group and control group, with 30 cases in each group. Mean arterial pressure (MAP), heart rate (HR), arterial partial pressure of oxygen (PO2), and arterial partial pressure of nitrogen dioxide (PCO2) were recorded at the time points after anesthesia induction and before OLV (T1), OLV 30 min (T2), OLV 60 min (T3), OLV 120 min (T4), OLV end before (T5) and before leaving the room (T6) in both groups. Reverse Transcription-Polymerase Chain Reaction (RT-qPCR) was applied to detect the levels of CC16 mRNA. Enzyme-linked immunosorbent assay (ELISA) was used to detect serum CC16 protein levels. The content of malondialdehyde (MDA) in serum was determined by thio barbituric acid (TBA) method. ELISA was used to measure the concentrations of TNF-α (tumor necrosis factor-alpha)/and IL-6 (interleukin 6).

Results: DEX treatment slowed down HR at time points T1-T6 and increased PO2 and PCO2 at time points T2-T5 compared with the control group. Moreover, at time points T2-T6, DEX treatment reduced the levels of club cell secretory protein-16 (CC16) mRNA and serum CC16 protein levels. Furthermore, DEX treatment caused the reduction of MDA, TNF-α and IL-6 concentrations in serum of patients.

Conclusion: During the OLV process, DEX could reduce serum CC16 protein levels, inhibit inflammatory reactions and oxidative stress, and improve oxygenation index, indicating a protective effect on lung injury during OLV.

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右美托咪定通过抑制炎症反应和氧化应激改善食管癌患者单肺通气后的肺损伤。
目的:探讨右美托咪定(DEX)对单肺通气(OLV)患者肺损伤的影响:方法:将接受OLV全身麻醉的食管癌患者随机分为DEX组和对照组,每组30例。记录两组患者麻醉诱导后至OLV前(T1)、OLV 30 min(T2)、OLV 60 min(T3)、OLV 120 min(T4)、OLV结束前(T5)和离开病房前(T6)的平均动脉压(MAP)、心率(HR)、动脉血氧分压(PO2)和动脉血二氧化氮分压(PCO2)。应用反转录聚合酶链反应(RT-qPCR)检测 CC16 mRNA 的水平。酶联免疫吸附试验(ELISA)用于检测血清 CC16 蛋白水平。用硫代巴比妥酸(TBA)法测定血清中丙二醛(MDA)的含量。ELISA法测定TNF-α(肿瘤坏死因子-α)/IL-6(白细胞介素6)的浓度:结果:与对照组相比,DEX治疗在T1-T6时间点减缓了HR,在T2-T5时间点增加了PO2和PCO2。此外,在T2-T6时间点,DEX治疗降低了会厌细胞分泌蛋白-16(CC16)mRNA水平和血清CC16蛋白水平。此外,DEX治疗还能降低患者血清中MDA、TNF-α和IL-6的浓度:结论:在OLV过程中,DEX可降低血清CC16蛋白水平,抑制炎症反应和氧化应激,改善氧合指数,对OLV过程中的肺损伤有保护作用。
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来源期刊
Toxicology Research
Toxicology Research TOXICOLOGY-
CiteScore
3.60
自引率
0.00%
发文量
82
期刊介绍: A multi-disciplinary journal covering the best research in both fundamental and applied aspects of toxicology
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