Astaxanthin Induces Apoptosis in Human Osteosarcoma MG-63 Cells.

IF 1.1 4区 医学 Q3 BIOLOGY Folia Biologica Pub Date : 2023-01-01 DOI:10.14712/fb2023069050186
Guangyu Wang, Xu Tian, Lintao Liu, Jingming Dong
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Abstract

We explored the mechanism of human osteosarcoma MG-63 cell apoptosis induced by asta-xanthin. The MTT assay was used to detect the effect of astaxanthin on cell viability. Morphological changes associated with apoptosis were observed after DAPI staining. Early and late stages of apoptosis were detected by flow cytometry with annexin V-FITC/PI staining. Activation of caspases-8, -9 and -3 was detected by enzyme activity in vitro. Changes in the mitochondrial membrane potential were detected by MitoCapture staining. Western blot was used to detect the cleavage of PARP, which is a caspase-3 substrate, the release of cytochrome c and Smac into the cytosol, the translocation of pro-apoptotic proteins Bax and Bak, and the expression of mitochondrial pathway-related proteins. The translocation of Bax was also detected by immunofluorescence assay. Astaxanthin significantly inhibited the viability of human osteosarcoma MG-63 cells with an IC50 value of 12.36 μg/ml. The DAPI-stained cells showed characteristic apoptotic morphological changes - cell shrinkage, cell membrane blebbing, nuclear condensation, and apoptotic body formation. Cytochrome c and Smac were released from mitochondria to the cytosol. Pro-apoptotic proteins Bax and Bak were rapidly translocated to mitochondria after six hours of astaxanthin action. Caspases-9 and -3 were activated and PARP was cleaved. The expression of anti-apoptotic proteins Bcl-2, Bcl-xL and XIAP was significantly decreased. Astaxanthin induced human osteosarcoma MG-63 cell apoptosis through the mitochondria-mediated endogenous apoptosis pathway.

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虾青素诱导人骨肉瘤 MG-63 细胞凋亡
我们探讨了虾青素诱导人骨肉瘤MG-63细胞凋亡的机制。MTT试验用于检测虾青素对细胞活力的影响。DAPI染色后观察到与细胞凋亡相关的形态变化。用流式细胞仪和附件素 V-FITC/PI 染色法检测细胞凋亡的早期和晚期阶段。体外酶活性检测了 caspases-8、-9 和-3 的活化。线粒体膜电位的变化通过 MitoCapture 染色法检测。用 Western 印迹法检测了作为 caspase-3 底物的 PARP 的裂解、细胞色素 c 和 Smac 向细胞质的释放、促凋亡蛋白 Bax 和 Bak 的转位以及线粒体通路相关蛋白的表达。免疫荧光试验也检测到了 Bax 的转位。虾青素能明显抑制人骨肉瘤 MG-63 细胞的活力,IC50 值为 12.36 μg/ml。DAPI染色的细胞表现出特征性的凋亡形态学变化--细胞萎缩、细胞膜出血、核凝聚和凋亡体形成。细胞色素 c 和 Smac 从线粒体释放到细胞膜。在虾青素作用六小时后,促凋亡蛋白 Bax 和 Bak 被迅速转运到线粒体。Caspases-9和-3被激活,PARP被裂解。抗凋亡蛋白 Bcl-2、Bcl-xL 和 XIAP 的表达明显减少。虾青素通过线粒体介导的内源性凋亡途径诱导人骨肉瘤MG-63细胞凋亡。
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来源期刊
Folia Biologica
Folia Biologica 医学-生物学
CiteScore
1.40
自引率
0.00%
发文量
5
审稿时长
3 months
期刊介绍: Journal of Cellular and Molecular Biology publishes articles describing original research aimed at the elucidation of a wide range of questions of biology and medicine at the cellular and molecular levels. Studies on all organisms as well as on human cells and tissues are welcome.
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