Abundance of Colistin-Resistance Genes in Retail Meats in Vietnam.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Foodborne pathogens and disease Pub Date : 2024-08-01 Epub Date: 2024-05-03 DOI:10.1089/fpd.2023.0162

Yen Hai Le, Kanoko Ikawa, Hoa Thi Thanh Hoang, Hatsue Isomura, Diep Thi Khong, Thang Nam Nguyen, Tram Anh Que, Dung Tien Pham, Kaori Tanaka, Yoshimasa Yamamoto

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Abstract

The degree of contamination of retail meat with colistin-resistant bacteria and its potential contribution to dissemination within communities remains to be determined. Thus, we aimed to elucidate the contamination status of colistin-resistance genes, indicative of colistin-resistant bacteria, in retail meats in Vietnam. In total, 46 chicken and 49 pork meats from stores in Vietnam and Japan were examined. Multiplex real-time polymerase chain reaction with TaqMan probes was performed for detecting mcr-1, mcr-3, and Escherichia coli 16S rRNA. Colistin-resistant bacteria in meats were isolated using selective media. The minimum inhibitory concentrations of colistin were determined using the broth microdilution method. The results showed that 70.7% of chicken meats in Vietnam were contaminated with both mcr-1 and mcr-3. Meanwhile, mcr-1 and mcr-3 were detected in 15.9% and 40.9% of pork meat, respectively. Only mcr-3 was detected in 40% of chicken in Japan. In addition, mcr-1-harboring E. coli and mcr-3-harboring Aeromonas were isolated from chicken meats in Vietnam. Some of these isolates showed colistin resistance. These results showed that most retail meats were highly contaminated with colistin-resistance genes. Notably, our results suggest that mcr-3 is more prevalent in the contaminated samples compared with mcr-1.

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越南零售肉类中耐药基因的丰度。

零售肉类受耐药细菌污染的程度及其对社区内传播的潜在影响仍有待确定。因此，我们的目的是阐明越南零售肉类中耐药基因的污染状况，这些基因表明存在耐药细菌。我们总共检测了来自越南和日本商店的 46 种鸡肉和 49 种猪肉。使用 TaqMan 探针进行了多重实时聚合酶链反应，以检测 mcr-1、mcr-3 和大肠杆菌 16S rRNA。使用选择性培养基分离了肉类中的耐大肠菌素细菌。使用肉汤微稀释法测定了大肠杆菌素的最低抑菌浓度。结果显示，越南 70.7% 的鸡肉同时受到 mcr-1 和 mcr-3 的污染。同时，在 15.9% 和 40.9% 的猪肉中分别检测到了 mcr-1 和 mcr-3。在日本，只有 40% 的鸡肉中检测到了 mcr-3。此外，越南从鸡肉中分离出了携带 mcr-1 的大肠杆菌和携带 mcr-3 的气单胞菌。其中一些分离物对可乐定有抗药性。这些结果表明，大多数零售肉类都受到耐可乐定基因的高度污染。值得注意的是，我们的结果表明，与 mcr-1 相比，mcr-3 在受污染的样本中更为普遍。

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.