Development of Chlorhexidine-loaded Lipid Nanoparticles Incorporated in a Bioceramic Endodontic Sealer

IF 3.5 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of endodontics Pub Date : 2024-08-01 DOI:10.1016/j.joen.2024.04.012

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Abstract

Introduction

This study aimed to assess BioRoot RCS (BR) incorporating liposomal chlorhexidine digluconate (CHX) for its antibacterial activity, drug release capacity, and physicochemical properties.

Methods

Drug release of CHX liposomal formulations in combination with BR was evaluated spectrophotometrically and through mathematical release models for 30 days. A selected combination was evaluated for antimicrobial properties against Enterococcus faecalis biofilm growth on human dentin. Cytotoxicity was assessed following the ISO 10993-5:2019 standard on days 1, 3, and 7. Physicochemical properties were evaluated through setting time, Fourier transform infrared spectroscopy, solubility, contact angle, and film thickness.

Results

From BR, liposomal CHX released up to 7-fold higher CHX than CHX solution (P < .05), following a triphasic drug release pattern compared to the CHX solution, which followed a quasi-Fickian diffusion. BR combined with a selected liposomal CHX completely inhibited E. faecalis biofilm growth compared to the combination of BR with CHX solution and the control group (P < .05). Liposomal CHX decreased the contact angle (P < .05) and solubility but increased cytotoxicity (P < .05) of BR, staying above the ISO threshold. None of the other physicochemical characteristics tested differed from BR (P > .05).

Conclusion

This liposomal formulation improved CHX release from BR, enhancing the antibacterial effectiveness. It presents a promising approach for local antibiofilm therapy in endodontics without substantially altering the physicochemical characteristics of BR.

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在生物陶瓷牙髓封闭剂中加入氯己定脂质纳米粒子的开发。

简介：本研究旨在评估含有二葡萄糖酸氯己定（CHX）脂质体的 BioRoot RCS（BR）的抗菌活性、药物释放能力和理化性质：方法：通过分光光度法和数学释放模型对 CHX 脂质体制剂与 BR 组合的药物释放进行了为期 30 天的评估。评估了所选组合对人牙本质上粪肠球菌（E. faecalis）生物膜生长的抗菌特性。根据 ISO 10993-5:2019 标准，在第 1、3 和 7 天对细胞毒性进行了评估。通过凝固时间、傅立叶变换红外光谱（FTIR）、溶解度、接触角和薄膜厚度对理化性质进行了评估：结果：从 BR 中，脂质体 CHX 的释放量是 CHX 溶液的 7 倍（p0.05）：结论：这种脂质体配方改善了 CHX 从 BR 中的释放，提高了抗菌效果。结论：这种脂质体制剂提高了 CHX 从 BR 中的释放率，增强了抗菌效果，为根管治疗中的局部抗生物膜疗法提供了一种很有前景的方法，而且不会大幅改变 BR 的理化特性。

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来源期刊

Journal of endodontics 医学-牙科与口腔外科

CiteScore

8.80

自引率

9.50%

发文量

224

审稿时长

42 days

期刊介绍： The Journal of Endodontics, the official journal of the American Association of Endodontists, publishes scientific articles, case reports and comparison studies evaluating materials and methods of pulp conservation and endodontic treatment. Endodontists and general dentists can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation in the one journal that helps them keep pace with rapid changes in this field.