Genotypic insights to Panton-Valentine leukocidin positive methicillin-resistant Staphylococcus aureus isolated from cattle mastitis.

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Journal of Dairy Research Pub Date : 2024-05-06 DOI:10.1017/S0022029924000244
Muhammad Ijaz, Nauman Zaheer Ghumman, Muhammad Umar Javed, Arslan Ahmed, Hamza Rasheed, Farwa Anwaar, Muhammad Jawad Sabir, Iqra Muzammil
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Abstract

In this research communication we investigate the prevalence and antimicrobial susceptibility of S. aureus harboring virulent genes responsible for mastitis in cattle of Punjab, Pakistan. A total of 690 milk samples were collected from commercial dairy farms for analysis of the prevalence of subclinical and clinical mastitis and isolation of S. aureus. Virulence ability and methicillin resistance in S. aureus (MRSA) was determined by targeting the pvl (the gene for Panton-Valentine leukocidin) and mecA genes, respectively. A total of 175 S. aureus isolates exhibiting prevalence of pvl gene (6.28%) and mecA gene (22.28%) were determined. Antimicrobial susceptibility testing of pvl positive and negative MRSA against different classes of antibiotics revealed 100% resistance against β-lactams while 100% sensitivity towards tylosin and linezolid.

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对从牛乳腺炎中分离出的潘通-瓦伦丁白细胞介素阳性耐甲氧西林金黄色葡萄球菌基因型的认识。
在这篇研究通讯中,我们调查了巴基斯坦旁遮普省牛群中金黄色葡萄球菌的流行情况和对抗菌药的敏感性,这些金黄色葡萄球菌含有导致乳腺炎的毒力基因。我们从商业奶牛场共采集了 690 份牛奶样本,用于分析亚临床和临床乳腺炎的流行情况以及金黄色葡萄球菌的分离情况。金黄色葡萄球菌(MRSA)的致病能力和耐甲氧西林性分别通过pvl(潘顿-瓦伦丁白细胞介素基因)和mecA基因来确定。共测定了 175 个金黄色葡萄球菌分离株,其 pvl 基因(6.28%)和 mecA 基因(22.28%)的流行率分别为 6.28%和 22.28%。pvl 阳性和阴性 MRSA 对不同种类抗生素的抗菌药敏感性测试显示,它们对 β-内酰胺类抗生素的耐药性为 100%,而对泰乐菌素和利奈唑胺的敏感性为 100%。
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来源期刊
Journal of Dairy Research
Journal of Dairy Research 农林科学-奶制品与动物科学
CiteScore
3.80
自引率
4.80%
发文量
117
审稿时长
12-24 weeks
期刊介绍: The Journal of Dairy Research is an international Journal of high-standing that publishes original scientific research on all aspects of the biology, wellbeing and technology of lactating animals and the foods they produce. The Journal’s ability to cover the entire dairy foods chain is a major strength. Cross-disciplinary research is particularly welcomed, as is comparative lactation research in different dairy and non-dairy species and research dealing with consumer health aspects of dairy products. Journal of Dairy Research: an international Journal of the lactation sciences.
期刊最新文献
In vitro antimicrobial and antibiofilm activity of phage cocktail against Mammaliicoccus sciuri, a causative agent of bovine mastitis. Relationship between body weight and hip width in dairy buffaloes (Bubalus bubalis). Effect of double-premilking teat disinfection protocols on bacterial counts on teat skin of cows and milker gloves in a free-stall-housed dairy herd. Investigating the relationship between heat load and shade seeking behaviour in dairy buffaloes. Genotypic insights to Panton-Valentine leukocidin positive methicillin-resistant Staphylococcus aureus isolated from cattle mastitis.
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