In Silico Identification and Characterization of Fatty Acid Desaturase (FAD) Genes in Argania spinosa L. Skeels: Implications for Oil Quality and Abiotic Stress.

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS Bioinformatics and Biology Insights Pub Date : 2024-05-05 eCollection Date: 2024-01-01 DOI:10.1177/11779322241248908
Abdelmoiz El Faqer, Karim Rabeh, Mohammed Alami, Abdelkarim Filali-Maltouf, Bouchra Belkadi
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Abstract

Fatty acid desaturase (FAD) is the key enzyme that leads to the formation of unsaturated fatty acids by introducing double bonds into hydrocarbon chains, and it plays a critical role in plant lipid metabolism. However, no data are available on enzyme-associated genes in argan trees. In addition, a candidate gene approach was adopted to identify and characterize the gene sequences of interest that are potentially involved in oil quality and abiotic stress. Based on phylogenetic analyses, 18 putative FAD genes of Argania spinosa L. (AsFAD) were identified and assigned to three subfamilies: stearoyl-ACP desaturase (SAD), Δ-12 desaturase (FAD2/FAD6), and Δ-15 desaturase (FAD3/FAD7). Furthermore, gene structure and motif analyses revealed a conserved exon-intron organization among FAD members belonging to the various oil crops studied, and they exhibited conserved motifs within each subfamily. In addition, the gene structure shows a wide variation in intron numbers, ranging from 0 to 8, with two highly conserved intron phases (0 and 1). The AsFAD and AsSAD subfamilies consist of three (H(X)2-4H, H(X)2-3HH, and H/Q (X)2-3HH) and two (EEN(K)RHG and DEKRHE) conserved histidine boxes, respectively. A set of primer pairs were designed for each FAD gene, and tested on DNA extracted from argan leaves, in which all amplicons of the expected size were produced. These findings of candidate genes in A spinosa L. will provide valuable knowledge that further enhances our understanding of the potential roles of FAD genes in the quality of oil and abiotic stress in the argan tree.

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对刺阿干树(Argania spinosa L. Skeels)脂肪酸去饱和酶(FAD)基因的硅学鉴定和特征描述:对油质和非生物压力的影响。
脂肪酸去饱和酶(FAD)是通过在碳氢链中引入双键形成不饱和脂肪酸的关键酶,在植物脂质代谢中起着至关重要的作用。然而,目前还没有关于阿甘树中酶相关基因的数据。此外,研究人员还采用了候选基因方法,以确定可能与油质和非生物胁迫有关的相关基因序列并描述其特征。根据系统发育分析,确定了 18 个假定的刺阿干树 FAD 基因(AsFAD),并将其归入三个亚家族:硬脂酰-ACP 去饱和酶(SAD)、Δ-12 去饱和酶(FAD2/FAD6)和Δ-15 去饱和酶(FAD3/FAD7)。此外,基因结构和基序分析表明,所研究的各种油料作物的 FAD 成员的外显子-内含子组织结构是一致的,并且在每个亚家族中都表现出一致的基序。此外,基因结构显示内含子数目差异很大,从 0 到 8 不等,其中有两个高度保守的内含子阶段(0 和 1)。AsFAD 和 AsSAD 亚家族分别由三个(H(X)2-4H、H(X)2-3HH 和 H/Q (X)2-3HH)和两个(EEN(K)RHG 和 DEKRHE)保守组氨酸框组成。研究人员为每个 FAD 基因设计了一组引物对,并对从坚果叶片中提取的 DNA 进行了测试,结果显示所有的扩增子都达到了预期的大小。这些关于 A spinosa L. 候选基因的发现将为我们提供宝贵的知识,进一步加深我们对 FAD 基因在坚果油品质和非生物胁迫中的潜在作用的理解。
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来源期刊
Bioinformatics and Biology Insights
Bioinformatics and Biology Insights BIOCHEMICAL RESEARCH METHODS-
CiteScore
6.80
自引率
1.70%
发文量
36
审稿时长
8 weeks
期刊介绍: Bioinformatics and Biology Insights is an open access, peer-reviewed journal that considers articles on bioinformatics methods and their applications which must pertain to biological insights. All papers should be easily amenable to biologists and as such help bridge the gap between theories and applications.
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