Detection of Methylene Tetrahydrofolate Reductase (MTHFR C677T) Mutation among Acute Lymphoblastic Leukemia in Sudanese Patients.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2023-10-01 DOI:10.61186/rbmb.12.3.458
Waad Almuatasem Mohieldeen, Albara Ahmed, Yousif Mohammed Elmosaad, Rania Saad Suliman, Abdulaziz Alfahed, Ahmed Hjazi, Humood Al Shmrany, Nora Hakami, Mohammed Ageeli Hakami, Alhomidi Almotiri, Hisham Ali Waggiallah
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Abstract

Background: A genetic polymorphism that causes abnormal folate metabolism may lead to genomic instability and increase susceptibility to malignancies such as Acute Lymphoblastic leukemia (ALL). The purpose of this research is to identify methylene tetrahydrofolate reductase (MTHFR C677T) (NCBI ID: 4524) mutation in ALL patients.

Methods: The study was a descriptive case-control hospital-based study with one hundred Sudanese participants divided equally into fifty (50) Sudanese ALL diagnosed patients as cases and fifty (50) Sudanese individuals as controls. The MTHFR C677T mutant allele was detected using conventional PCR, with the primer sequence of MTHFR C677T F-TGAAGGAAGGTGTCTGCGGGA R-AGGACGGTGCGGTGAGAGTG. The study was conducted from January to March 2023, and samples were collected from the Radiation and Isotops Center at Khartoum Hospital.

Results: The investigation revealed that 12 of the 50 patients in the case group (24%) had the MTHFR C677T mutant allele, and the study also revealed that there is significant correlation with the control group. There is no significant relationship between socio-demographic variables and MTHFR mutation detection in ALL patients. Also, the sociodemographic variables predictors of MTHFR mutation among ALL patients adjusted for smoking habit revealed no significant relationship.

Conclusion: According to the findings of this study, the mutant allele of the Methylene Tetra Hydro Folate Reductase C677T was detected and demonstrated varying degrees of significance. It was concluded that the MTHFR C677T gene mutation was associated with acute lymphoblastic leukemia in Sudanese patients.

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在苏丹急性淋巴细胞白血病患者中检测亚甲基四氢叶酸还原酶 (MTHFR C677T) 突变。
背景:导致叶酸代谢异常的基因多态性可能会导致基因组不稳定,并增加对急性淋巴细胞白血病(ALL)等恶性肿瘤的易感性。本研究旨在确定ALL患者的亚甲基四氢叶酸还原酶(MTHFR C677T)(NCBI ID:4524)突变:该研究是一项以医院为基础的病例对照描述性研究,100 名苏丹参与者平均分为 50 名苏丹 ALL 诊断患者作为病例,50 名苏丹人作为对照。采用常规 PCR 方法检测 MTHFR C677T 突变等位基因,引物序列为 MTHFR C677T F-TGAAGGAAGGTGTCTGCGGGA R-AGGACGGTGCGGTGAGTG。研究于 2023 年 1 月至 3 月进行,样本从喀土穆医院辐射和同位素中心采集:调查显示,病例组的 50 名患者中有 12 人(24%)具有 MTHFR C677T 突变等位基因,研究还显示,这与对照组存在显著相关性。社会人口学变量与 ALL 患者的 MTHFR 突变检测之间无明显关系。此外,社会人口学变量预测ALL患者MTHFR突变与吸烟习惯的关系也不明显:根据这项研究的结果,亚甲基四氢叶酸还原酶 C677T 突变等位基因被检测到,并显示出不同程度的重要性。结论是,MTHFR C677T 基因突变与苏丹患者的急性淋巴细胞白血病有关。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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