Wound-healing effect of topical nanoemulsion-loaded cream and gel formulations of Hippophae rhamnoides L. (sea buckthorn) fruit oil and their acute dermal toxicity study on female SD rats.

IF 1.4 4区医学 Q4 PHARMACOLOGY & PHARMACY Indian Journal of Pharmacology Pub Date : 2024-03-01 Epub Date: 2024-04-30 DOI:10.4103/ijp.ijp_370_23

Dattatraya Dinkar Gore, Nisha Sharma, Nidhi Mishra, Prashantkumar K Parmar, Soni Ranjana, Dinesh Kumar, Sanjay M Jachak, Gopabandhu Jena, Kulbhushan Tikoo, Arvind K Bansal, Inder Pal Singh

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Abstract

Objective: The objective of this study was to evaluate the efficacy and safety of topical nanoemulsion (NE)-loaded cream and gel formulations of Hippophae rhamnoides L. (sea buckthorn [SBT]) fruit oil for wound healing.

Materials and methods: The NE-loaded cream and gel formulations of H. rhamnoides L. (SBT) fruit oil (IPHRFH) were prepared and evaluated for their wound-healing activity on female Sprague-Dawley (SD) rats. They were further divided into groups (seven) and the wound-healing activity was determined by measuring the area of the wound on the wounding day and on the 0th, 4th, 8th, and 10th days. The acute dermal toxicity of the formulations was assessed by observing the erythema, edema, and body weight (BW) of the rats.

Results: The topical NE cream and gel formulations of H. rhamnoides L. (SBT) fruit oil showed significant wound-healing activity in female SD rats. The cream formulation of IPHRFH showed 78.96%, the gel showed 72.59% wound contraction on the 8th day, whereas the positive control soframycin (1% w/w framycetin) had 62.29% wound contraction on the 8th day. The formulations also showed a good acute dermal toxicity profile with no changes significantly affecting BW and dermal alterations.

Conclusions: The results of this study indicate that topical NE-loaded cream and gel formulation of H. rhamnoides L. (SBT) fruit oil are safe and effective for wound healing. The formulations showed no signs of acute dermal toxicity in female SD rats.

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沙棘果油纳米乳剂和凝胶外用制剂的伤口愈合效果及其对雌性 SD 大鼠的急性皮肤毒性研究。

研究目的本研究旨在评估纳米乳液（NE）负载的沙棘果油乳膏和凝胶制剂对伤口愈合的有效性和安全性：制备了含 NE 的沙棘果油乳膏和凝胶制剂（IPHRFH），并对其在雌性 Sprague-Dawley (SD) 大鼠身上的伤口愈合活性进行了评估。大鼠被分为七组，伤口愈合活性通过测量伤口面积来确定，测量方法包括伤口当天、第 0 天、第 4 天、第 8 天和第 10 天的伤口面积。通过观察大鼠的红斑、水肿和体重（BW）来评估制剂的急性皮肤毒性：结果：鼠李（SBT）果油的局部 NE 霜和凝胶配方对雌性 SD 大鼠显示出显著的伤口愈合活性。第 8 天，IPHRFH 乳霜制剂的伤口收缩率为 78.96%，凝胶制剂的伤口收缩率为 72.59%，而阳性对照 Soframycin（1% w/w framycetin）的伤口收缩率为 62.29%。这些制剂还显示出良好的急性皮肤毒性，对体重和皮肤改变没有显著影响：本研究结果表明，鼠李（SBT）果油的局部 NE 负荷乳膏和凝胶配方对伤口愈合安全有效。这些制剂对雌性 SD 大鼠未显示出急性皮肤毒性。

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来源期刊

Indian Journal of Pharmacology 医学-药学

CiteScore

4.00

自引率

4.20%

发文量

审稿时长

4-8 weeks

期刊介绍： Indian Journal of Pharmacology accepts, in English, review articles, articles for educational forum, original research articles (full length and short communications), letter to editor, case reports and interesting fillers. Articles concerning all aspects of pharmacology will be considered. Articles of general interest (e.g. methods, therapeutics, medical education, interesting websites, new drug information and commentary on a recent topic) are also welcome.