Cryopreservation of bovine semen using extract of Cinnamomum zeylanicum.

IF 1 4区生物学 Q3 BIOLOGY Cryo letters Pub Date : 2024-05-01

K Alam, S Srivastava, B Singh, Saurabh EmptyYN Y, R Kumar, R Kumar, D S Sakhahari

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引用次数: 0

Abstract

Background: Antioxidants minimise oxidative stress and enhance sperm quality in the process of cryopreservation.

Objective: To assess the impact of Cinnamomum zeylanicum extract as an additive during the post-dilution and post-thaw stages of Murrah buffalo semen cryopreservation.

Materials and methods: The semen sample was diluted using Tris-Egg-Yolk-Citric-Acid-Fructose-Glycerol extender and subsequently divided into three groups: Group 1, TEYCAFG without any additives or controls (C); Group 2, TEYCAFG fortified with a 50 ug/mL aqueous extract of cinnamon (T1); and Group 3, TEYCAFG fortified with a 50 ug/mL ethanolic extract of cinnamon (T2). The evaluation included an assessment of progressive motility, live spermatozoa, sperm abnormalities, HOST, CMPT, and enzyme leakage (GOT and GPT) at both the post-dilution and post-thaw stages.

Results: The groups that received cinnamon supplementation demonstrated statistically significant improvements (p<0.05) in various parameters, including an increase in the progressive motility, live spermatozoa, and HOS-positive spermatozoa, as well as greater distance traveled by vanguard spermatozoa compared to the control group. Furthermore, the cinnamon-added groups exhibited a significant decrease (p<0.05) in the percentage of sperm abnormalities and lower enzyme leakage (GOT and GPT) in post-thawed semen.

Conclusion: Aqueous extract of C. zeylanicum at a concentration of 50 µg/mL provides superior protection of sperm structures and functions as compared to both the ethanolic extract of C. zeylanicum at the same concentration and the control group. Doi.org/10.54680/fr24310110712.

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使用肉桂提取物对牛精液进行冷冻保存。

背景：抗氧化剂可在冷冻保存过程中减少氧化应激并提高精子质量：评估肉桂提取物作为添加剂在穆拉水牛精液冷冻保存的稀释后和解冻后阶段的影响：使用三蛋黄-柠檬酸-果糖-甘油扩展剂稀释精液样本，然后将其分为三组：第1组为不含任何添加剂或对照组的TEYCAFG（C）；第2组为添加了50微克/毫升肉桂水提取物的TEYCAFG（T1）；第3组为添加了50微克/毫升肉桂乙醇提取物的TEYCAFG（T2）。评估包括稀释后和解冻后阶段的精子活力、活精子、精子畸形、HOST、CMPT和酶渗漏（GOT和GPT）：结果：补充肉桂的组别在统计学上有显著改善（p与相同浓度的肉桂乙醇提取物和对照组相比，浓度为 50 µg/mL 的肉桂水提取物能更好地保护精子的结构和功能。Doi.org/10.54680/fr24310110712.

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.