Antibiotic Susceptibility and Biofilm Production among Coagulase Negative Staphylococci Isolated from Clinical Samples at Tertiary Care Hospital.

Pradeep Kumar Shah, Niru Bhandari, Basanta Tamang, Rajendra D Joshi
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Abstract

Background: Coagulase Negative Staphylococci have been widely associated with medical device implant treatment and immune-compromised patients. Despite having increasing interest in Coagulase Negative Staphylococci, few studies from Nepal have reported the association of these organisms with urinary tract infections, conjunctivitis, high vaginal swabs, and cerebrospinal fluid. This study was carried out to determine antibiotic susceptibility pattern and biofilm production among Coagulase Negative Staphylococci isolated from clinical samples at tertiary care hospital.

Methods: This study was a hospital based cross-sectional study in which 3690 clinical samples were included. Isolation and identification of isolates was done following standard microbiological protocol. Coagulase Negative Staphylococci were identified phenotypically on the basis of gram staining, slide and tube coagulase test and by various sugar fermentation tests. Antibiotic susceptibility test was done following Kirby Bauer disk diffusion method (Clinical and Laboratory Standards Institute 2020). Biofilm production was determined by Tissue Culture Plate technique.

Results: A total of 113 isolates of Coagulase Negative Staphylococci were detected. Among them S. epidermidis (45.1%), S. saprophyticus (23.9%), S. haemolyticus (16.8%), S. hominis (5.3%), S. capitis (2.7%), -----S. cohini (1.8%), S. lugdunensis (1.8%) and S. sciuri (2.7%) were identified phenotypically. All isolates were found to be resistant against Ampicillin and 111 (98.2%) were sensitive against Linezolid.23.9% of CoNS were strong biofilm producers, 19.5% moderate and 56.6 % were non/weak biofilm producers.

Conclusions: It requires susceptibility test for prescribing antibiotics against Coagulase Negative Staphylococci in hospital and the misuse of antibiotics should be prevented.

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三级甲等医院临床样本中分离出的凝固酶阴性葡萄球菌的抗生素敏感性和生物膜生成。
背景:凝固酶阴性葡萄球菌与医疗器械植入治疗和免疫力低下的患者广泛相关。尽管人们对凝固酶阴性葡萄球菌的兴趣与日俱增,但尼泊尔很少有研究报告称这些微生物与尿路感染、结膜炎、高浓度阴道拭子和脑脊液有关。本研究旨在确定从三级医院临床样本中分离出的凝固酶阴性葡萄球菌的抗生素敏感性模式和生物膜生成情况:本研究是一项基于医院的横断面研究,共纳入 3690 份临床样本。分离和鉴定按照标准微生物学方案进行。根据革兰氏染色、玻片和试管凝固酶试验以及各种糖发酵试验对凝固酶阴性葡萄球菌进行表型鉴定。抗生素药敏试验采用柯比鲍尔盘扩散法(临床与实验室标准研究所,2020 年)进行。生物膜的产生采用组织培养板技术进行测定:结果:共检测到 113 株凝固酶阴性葡萄球菌。其中表皮葡萄球菌(45.1%)、溶血性葡萄球菌(23.9%)、溶血性葡萄球菌(16.8%)、同形葡萄球菌(5.3%)、头炎葡萄球菌(2.7%)、-----S. cohini(1.8%)、卢格登氏葡萄球菌(1.8%)和斯库里葡萄球菌(2.7%)已被表型鉴定。23.9% 的 CoNS 具有较强的生物膜生成能力,19.5% 为中等水平,56.6% 为非/弱生物膜生成能力:医院在开具针对凝固酶阴性葡萄球菌的抗生素处方时需要进行药敏试验,并应防止滥用抗生素。
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1.40
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期刊介绍: The journal publishes articles related to researches done in the field of biomedical sciences related to all the discipline of the medical sciences, medical education, public health, health care management, including ethical and social issues pertaining to health. The journal gives preference to clinically oriented studies over experimental and animal studies. The Journal would publish peer-reviewed original research papers, case reports, systematic reviews and meta-analysis. Editorial, Guest Editorial, Viewpoint and letter to the editor are solicited by the editorial board. Frequently Asked Questions (FAQ) regarding manuscript submission and processing at JNHRC.
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