Co-occurring microflora and mucin drive Pseudomonas aeruginosa diversification and pathoadaptation.

IF 5.1 Q1 ECOLOGY ISME communications Pub Date : 2024-03-28 eCollection Date: 2024-01-01 DOI:10.1093/ismeco/ycae043
Michael J Bottery, Helle Krogh Johansen, Jon W Pitchford, Ville-Petri Friman
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Abstract

While several environmental factors contribute to the evolutionary diversification of the pathogenic bacterium Pseudomonas aeruginosa during cystic fibrosis lung infections, relatively little is known about the impact of the surrounding microbiota. By using in vitro experimental evolution, we show that the presence of Stenotrophomonas maltophilia, Staphylococcus aureus, or them both, prevent the evolution of loss of virulence, which repeatedly occurs in the absence of these species due to mutations in regulators of the Pseudomonas Quinolone Signal quorum sensing system, vqsM and pqsR. Moreover, the strength of the effect of co-occurring species is attenuated through changes in the physical environment by the addition of mucin, resulting in selection for phenotypes resembling those evolved in the absence of the co-occurring species. Together, our findings show that variation in mucosal environment and the surrounding polymicrobial environment can determine the evolutionary trajectory of P. aeruginosa, partly explaining its diversification and pathoadaptation from acute to chronic phenotype during cystic fibrosis lung infections.

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共生微生物菌群和粘蛋白驱动铜绿假单胞菌的多样化和病理适应。
在囊性纤维化肺部感染期间,多种环境因素导致了致病菌铜绿假单胞菌的进化多样化,但人们对周围微生物群的影响却知之甚少。通过体外实验进化,我们发现嗜麦芽糖血单胞菌、金黄色葡萄球菌或它们两者的存在能阻止毒力丧失的进化,而在没有这些物种的情况下,由于假单胞菌喹诺酮信号法定量传感系统的调控因子 vqsM 和 pqsR 发生突变,毒力丧失会反复发生。此外,由于添加了粘蛋白,物理环境发生了变化,从而削弱了共生物种效应的强度,导致选择了与不存在共生物种时进化出的表型相似的表型。总之,我们的研究结果表明,粘膜环境和周围多微生物环境的变化可以决定铜绿假单胞菌的进化轨迹,从而部分解释了铜绿假单胞菌在囊性纤维化肺部感染过程中从急性表型到慢性表型的多样化和病理适应。
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